Welcome to Chinese Agricultural Science Bulletin,

Chinese Agricultural Science Bulletin ›› 2020, Vol. 36 ›› Issue (12): 62-67.doi: 10.11924/j.issn.1000-6850.casb20191100862

Previous Articles     Next Articles

Two Strains of Topramezone Degrading Fungi: Isolation and Identification

Pan Guoqiang1,2,3, Yang Fengshan1,2,3, Liu Xiaoxin1,2,3, Ma Yukun1,2,3, Fu Haiyan1,2,3, Liu Chunguang1,2,3()   

  1. 1Engineering Research Center of Agricultural Microbiology Technology, Ministry of Education, Heilongjiang University, Harbin 150500
    2Heilongjiang Provincial Key Laboratory of Ecological Restoration and Resource Utilization for Cold Region, School of Life Sciences, Heilongjiang University, Harbin 150080
    3Key Laboratory of Microbiology, College of Heilongjiang, Heilongjiang University, Harbin 150080
  • Received:2019-11-20 Revised:2019-12-28 Online:2020-04-25 Published:2020-04-21
  • Contact: Chunguang Liu E-mail:2005013@hlju.edu.cn

Abstract:

To solve the problem of a large number of residues in soil caused by the long-term application of corn field herbicide topramezone, the enrichment and acclimation method was used to screen the strains, and the high efficiency liquid chromatography method was used to determine the degradation ability, and the species were identified by morphology and 18S rDNA sequence analysis in this study. The results showed that five fungal strains TOF1, TOF2, TOF3, TOF4 and TOF5, which could grow with topramezone as the sole carbon source, were isolated and purified from the soil using topramezone for a long time. The degradation efficiency of initial concentration 400 mg/L topramezone was 35.86%, 34.35%, 32.12%, 9.91% and 27.99% in seven days, respectively. TOF1 and TOF2 were found to be highly repeatable, through repeated degradation validation. These two strains were identified as Penicillium chrysogenum and Penicillium tardochrysogenum. The acquisition of these two species provides new resources for bioremediation of contaminated soil with topramezone.

Key words: topramezone, fungus, isolation, identification, degradation

CLC Number: