Antibacterial Mechanism of Active Substances from Laminaria japonica Petroleum Ether Phase Against Clavibacter michiganensis subsp. michiganensis

doi:10.11924/j.issn.1000-6850.casb20191200930

Abstract

Abstract:

The aim is to clarify the mechanism of Laminaria japonica active substances inhibiting Clavibacter michiganensis subsp. michiganensis, and to provide a theoretical basis for the development of green antibacterial agents. Antibacterial components of L. japonica were detected by the tracking method. The study of the mechanism was carried out from multiple perspectives, including electron microscopy (SEM), membrane potential, reactive oxygen species, soluble proteins, and cell cycle. The results showed that the petroleum ether phase of L. japonica had the best bacteriostatic effect (P<0.05), and the diameter of inhibitory zone was 26.00 mm. The study of mechanism showed that cell morphology of C. michiganensis subsp. michiganensis changed significantly, the potential of membrane decreased by 17.49%-56.60%, the content of intracellular active oxygen increased by 1.19-3.98 times, intracellular protein content reduced significantly (P<0.05), and the proportion of C. michiganensis subsp. michiganensis in the cell division stage reduced significantly. The results indicate that active substances of L. japonica mainly act on the cell membrane, causing accumulation of free radicals, blocking normal passage, and ultimately achieving the effect of killing bacteria.

Key words: Clavibacter michiganensis subsp. michiganensis, Laminaria japonica active substance, scanning electron microscope, flow cytometer, antibacterial mechanism, cell membrane

CLC Number:

Du Beibei, Xie Shulian, Wang Mengliang, Cai Jin. Antibacterial Mechanism of Active Substances from Laminaria japonica Petroleum Ether Phase Against Clavibacter michiganensis subsp. michiganensis[J]. Chinese Agricultural Science Bulletin, 2020, 36(33): 127-133.

Figures/Tables 6

References 28

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