Photosynthetic Characteristics and Chromosome Karyotypes of Two Wild Soybean Varieties: Comparison at Different Growth Stages

doi:10.11924/j.issn.1000-6850.casb2020-0565

Abstract

Abstract:

To obtain high light efficiency of wild soybean materials and understand their best growth conditions, photosynthetic characteristics and chromosomal karyotype analysis of Dingpashan (Dingdou) and Kaili University (Kaidou) wild soybean at different growth stages were studied. The results show that Dingdou’s chlorophyll content at the flowering stage and the pod setting stage is lower than that of Kaidou. Dingdou’s chlorophyll content in the pod setting stage is significantly higher than that of the vegetative stage and the flowering stage. Kaidou’s chlorophyll content at the flowering stage and the pod setting stage is significantly higher than that at the vegetative stage. The photosynthetic rate, stomatal conductance, intercellular CO₂ and transpiration rate of Kaidou are all higher than those of Dingdou. The photosynthetic rate and stomatal conductance of Kaidou are both higher at the pod setting stage than that at the flowering stage and the vegetative stage. The photosynthetic rate of Dingdou is higher at the flowering stage than that at the vegetative stage and the pod setting stage. The intercellular CO₂concentration of Kaidou shows a decreasing trend at the pod setting stage, while the intercellular CO₂ concentration in the vegetative stage and the flowering stage has a decreasing trend first and then an increasing trend.The intercellular CO₂ concentration at Dingdou’s vegetative stage shows a trend of first decreasing then increasing and decreasing again, that at its flowering stage shows a trend of decreasing, and at the pod setting stage has a bimodal curve. The transpiration rate of the two wild soybean varieties is higher at pod setting stage than that at the flowering stage and the vegetative stage. Dingdou’s LCP is higher at the pod setting stage than that at the flowering stage and the vegetative stage, while LSP and P_max are higher at the flowering stage than that at the pod setting stage and the vegetative stage. The karyotype formula of Dingdou is 2n=24m+12sm+ 4st; the karyotype formula of Kaidou is 2n=22m+ 16sm + 2st. Therefore, the photosynthetic efficiency of Kaidou is higher than that of Dingdou, and the photosynthetic efficiency of both the two varieties are higher at the flowering stage than that at the pod setting stage and the vegetative stage. Dingdou’s chromosome type is 2B, and Kaidou is 2C.

Key words: wild soybean, photosynthetic characteristics, light response curve, karyotype analysis

CLC Number:

S565.1

Tian Xin, Zhong Cheng, Luo Huan, Li Xuepiao. Photosynthetic Characteristics and Chromosome Karyotypes of Two Wild Soybean Varieties: Comparison at Different Growth Stages[J]. Chinese Agricultural Science Bulletin, 2021, 37(27): 32-38.

Figures/Tables 10

References 30

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PAR/[μmol/(m²·s)]	V25-Pn/[μmol/(m²·s)]		R3-Pn/[μmol/(m²·s)]		R5-Pn/[μmol/(m²·s)]
PAR/[μmol/(m²·s)]	丁豆	凯豆	丁豆	凯豆	丁豆	凯豆
0	-0.6827	-1.4225	-0.7529	-0.7749	-0.8810	-1.4072
20	0.7807	0.5269	0.4610	0.3941	0.5952	0.3729
50	2.0747	2.5050	1.5989	1.3280	1.9366	1.9376
100	4.0974	4.4593	3.6500	2.6467	3.8208	4.1088
200	5.3105	5.9800	5.2742	7.2110	5.7061	7.6145
400	6.7687	10.8114	7.5497	11.5366	7.8741	11.4563
800	7.9696	13.9560	10.2199	16.3491	9.9025	15.5567
1200	8.6306	14.8648	11.3047	17.8705	10.6866	17.8271
1600	8.0092	15.1412	11.8100	20.1412	11.1006	17.6903
2000	7.8777	14.8488	11.3395	19.6072	10.4052	17.0275

PAR/[μmol/(m²·s)]	V25-Pn/[μmol/(m²·s)]		R3-Pn/[μmol/(m²·s)]		R5-Pn/[μmol/(m²·s)]
PAR/[μmol/(m²·s)]	丁豆	凯豆	丁豆	凯豆	丁豆	凯豆
0	-0.6827	-1.4225	-0.7529	-0.7749	-0.8810	-1.4072
20	0.7807	0.5269	0.4610	0.3941	0.5952	0.3729
50	2.0747	2.5050	1.5989	1.3280	1.9366	1.9376
100	4.0974	4.4593	3.6500	2.6467	3.8208	4.1088
200	5.3105	5.9800	5.2742	7.2110	5.7061	7.6145
400	6.7687	10.8114	7.5497	11.5366	7.8741	11.4563
800	7.9696	13.9560	10.2199	16.3491	9.9025	15.5567
1200	8.6306	14.8648	11.3047	17.8705	10.6866	17.8271
1600	8.0092	15.1412	11.8100	20.1412	11.1006	17.6903
2000	7.8777	14.8488	11.3395	19.6072	10.4052	17.0275

名称	时期	LCP	LSP	P_max	Rd	R²
丁豆	营养期	8.5143	1067.6232	7.8613	0.5962	0.9949
	盛花期	10.9833	1502.2111	10.3656	0.5114	0.9965
	结荚期	11.3364	1323.1997	9.9716	0.6561	0.9972
凯豆	营养期	13.6770	1639.6078	15.2097	0.7858	0.9941
	盛花期	18.1563	2000.0000	19.2078	0.8892	0.9971
	结荚期	16.9639	1827.4400	18.9893	0.9811	0.9983

名称	时期	LCP	LSP	P_max	Rd	R²
丁豆	营养期	8.5143	1067.6232	7.8613	0.5962	0.9949
	盛花期	10.9833	1502.2111	10.3656	0.5114	0.9965
	结荚期	11.3364	1323.1997	9.9716	0.6561	0.9972
凯豆	营养期	13.6770	1639.6078	15.2097	0.7858	0.9941
	盛花期	18.1563	2000.0000	19.2078	0.8892	0.9971
	结荚期	16.9639	1827.4400	18.9893	0.9811	0.9983

名称	染色体数	类型	臂比(L/S)	核型公式	最长/最短	类型	不对称系数/%
丁豆	24	m	1.30~1.69	2n=24m+12sm+2st	2.71	2B	61.93
	12	sm	1.71~2.83
	4	st	3.13~3.40
凯豆	22	m	1.24~1.66	2n=22m+16sm+2st	5.32	2C	64.31
	16	sm	2.00~2.52
	2	st	3.17~3.40