The Degradation Efficiency of Trametes hirsuta 27-1 Laccase on Phenolic Acids

doi:10.11924/j.issn.1000-6850.casb2020-0770

Abstract

Abstract:

The study aims to investigate the degradation efficiency of phenolic acid compounds by Trametes hirsuta 27-1 laccase so as to provide a basis for the degradation of phenolic acids in bamboo forest. Ultra-high performance liquid chromatography was used. By measuring the change of phenolic acid content after bacterial solution treatment, we explored the degradation properties of 10 kinds of phenolic acid compounds by Trametes hirsuta 27-1 laccase. The results show that laccase could completely degrade vanillic acid, caffeic acid and sinapic acid; the degradation effect of syringic acid, ferulic acid and gentian acid are general and the degradation rate is about 50%; the degradation effect of protocatechuic acid is poor, the degradation rate could be 35%, and coumaric acid, p-hydroxybenzoic acid and chlorogenic acid could not be degraded. By studying the efficacy of Trametes hirsuta 27-1 laccase in the degradation of syringic acid under different conditions, it is found that, under the condition of pH 5, temperature of 30℃ and the initial concentration of syringic acid less than 100 mg/L, the strain could have high degradation ability of syringic acid. After ABTS solution is added to the degradation system as a mediator, the degradation effect is much better. At a concentration of 0.5 mmol/L, the degradation rate could reach 100%. In conclusion, the laccase produced by Trametes hirsuta 27-1 has good degradation efficiency on phenolic acids.

Key words: phenolic acid, Trametes hirsuta 27-1, laccase, bamboo forest, autotoxins, degradation efficiency, syringic acid

CLC Number:

Zhou Xin, Miao Xiaolei, Yu Xuejun. The Degradation Efficiency of Trametes hirsuta 27-1 Laccase on Phenolic Acids[J]. Chinese Agricultural Science Bulletin, 2021, 37(13): 35-41.

Figures/Tables 8

References 26

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化合物	标准曲线	R²	线性范围/(μg/L)
原儿茶酸	Y=4.2654X-1.9390	0.99985	0.064~1.280
龙胆酸	Y=2.1431X-2.3371	0.99984	0.021~0.820
对羟基苯甲酸	Y=3.9147X-3.6120	0.99985	0.032~0.640
绿原酸	Y=2.2957X-2.3821	0.99983	0.026~0.520
香草酸	Y=8.9511X-4.6955	0.99987	0.028~1.560
咖啡酸	Y=10.5631X-12.4554	0.99981	0.065~2.598
丁香酸	Y=2.4513X-2.6354	0.99984	0.015~0.542
对香豆酸	Y=6.3682X-4.7442	0.99985	0.047~1.231
阿魏酸	Y=2.9674X-4.3961	0.99986	0.021~1.328
芥子酸	Y=8.6588X-4.5453	0.99986	0.043~0.965

化合物	标准曲线	R²	线性范围/(μg/L)
原儿茶酸	Y=4.2654X-1.9390	0.99985	0.064~1.280
龙胆酸	Y=2.1431X-2.3371	0.99984	0.021~0.820
对羟基苯甲酸	Y=3.9147X-3.6120	0.99985	0.032~0.640
绿原酸	Y=2.2957X-2.3821	0.99983	0.026~0.520
香草酸	Y=8.9511X-4.6955	0.99987	0.028~1.560
咖啡酸	Y=10.5631X-12.4554	0.99981	0.065~2.598
丁香酸	Y=2.4513X-2.6354	0.99984	0.015~0.542
对香豆酸	Y=6.3682X-4.7442	0.99985	0.047~1.231
阿魏酸	Y=2.9674X-4.3961	0.99986	0.021~1.328
芥子酸	Y=8.6588X-4.5453	0.99986	0.043~0.965

液体	0 h	4 h	8 h	12 h	24 h
灭活酶液	100.00	100.00	99.84	99.62	98.41
粗酶液	100.00	98.44	54.79	47.41	47.32
等体积超纯水	100.00	100.00	100.00	100.00	100.00

液体	0 h	4 h	8 h	12 h	24 h
灭活酶液	100.00	100.00	99.84	99.62	98.41
粗酶液	100.00	98.44	54.79	47.41	47.32
等体积超纯水	100.00	100.00	100.00	100.00	100.00