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Chinese Agricultural Science Bulletin ›› 2020, Vol. 36 ›› Issue (27): 122-128.doi: 10.11924/j.issn.1000-6850.casb20200300268

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Three Strains of Fomesafen Degrading Fungi: Isolation and Identification

Pan Guoqiang1,2,3(), Yang Fengshan1,2,3, Wang Jiarui1,2,3, Tang Yuanhao1,2,3, Ma Yukun1,2,3, Fu Haiyan1,2,3, Liu Chunguang1,2,3()   

  1. 1Engineering Research Center of Agricultural Microbiology Technology, Ministry of Education, Heilongjiang University, Harbin 150500
    2Heilongjiang Provincial Key Laboratory of Ecological Restoration and Resource Utilization for Cold Region, School of Life Sciences, Heilongjiang University, Harbin 150080
    3Key Laboratory of Microbiology, College of Heilongjiang, Heilongjiang University, Harbin 150080
  • Received:2020-03-27 Revised:2020-05-17 Online:2020-09-25 Published:2020-09-23
  • Contact: Liu Chunguang E-mail:1435297256@qq.com;2005013@hlju.edu.cn

Abstract:

In order to solve the problem of fomesafen residues in soybean field caused by the long-term application of the herbicide fomesafen, the enrichment and acclimation method was used to screen the strains, and the high efficiency liquid chromatography method was used to determine the degradation ability, and the species were identified by morphological and 16S rDNA sequence analysis in this study. The results showed that three fungal strains FF1, FF2 and FF3, which grew with fomesafen as the only carbon source, were isolated and purified from the soil with long-term application of fomesafen. Their degradation efficiency of the initial concentration 600 mg/L fomesafen in seven days was 21.03%, 15.74% and 11.88%, respectively. These three strains were identified as Aspergillus jensenii, Penicillium dipodomyicola and Rhizopus oryzae. The acquisition of the three species could provide new resources for bioremediation of contaminated soil with fomesafen.

Key words: fomesafen, fungus, isolation, identification, degradation

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