Effects of Three Debonding Reagents on the Hatching of Fertilized Eggs of Mastacembelus armatus

doi:10.11924/j.issn.1000-6850.casb2021-0406

Abstract

Abstract:

This paper aims to find a rapid and effective method of debonding and improve the efficiency of artificial breeding of Mastacembelus armatus. Yellow-earth slurry (A), talcum powder (B) and papain (C) were used for debonding fertilized eggs of Mastacembelus armatus, and the papain reagent was formulated in four recipes (C₁, C₂, C₃, C₄), and four debonding time (15s, 30s, 45s, 60s) were set, then the effects of three debongding reagents on hatching were assessed. The results showed the effect of talcum powder was better than that of yellow-earth slurry, but both of them were not good, the hatch rate of A group and B group was 30.71%±4.41% and 60.18%±10.12%, respectively. Papain was a new debonding reagent, the highest hatching rate was C₃-15s group, which was 96.26%±4.61%, followed by C₃-30 s group, and the hatching rate was 92.12%±3.32%. Therefore, the hatching rate of the fertilized eggs can be effectively improved by controlling the debonding recipe and time appropriately, and the optimal debonding recipe is Na₂SO₃ (0.25%) +L-cysteine monohydrochloride (0.125%) + papain (0.05%), the optimal debonding time is 15-30 s.

Key words: Mastacembelus armatus, fertilized egg, adhesive egg, debonding, yellow-earth slurry, talcum powder, papain

LIU Yinhua. Effects of Three Debonding Reagents on the Hatching of Fertilized Eggs of Mastacembelus armatus[J]. Chinese Agricultural Science Bulletin, 2023, 39(14): 133-138.

Figures/Tables 6

References 38

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脱黏液配方	配制方法
A	1.00 kg黄泥搓碎后，加5.00 kg水浸泡6~8 h，搅匀用40目筛绢滤渣备用
B	200.00 g医用滑石粉和25.00 g食盐，加水5.00 kg搅匀备用
C₁	50.00 g Na₂SO₃，25.00 g L-半胱氨酸盐酸盐和10.00 g木瓜酶，加5.00 kg水搅匀备用
C₂	25.00 g Na₂SO₃，12.50 g L-半胱氨酸盐酸盐和5.00 g木瓜酶，加5.00 kg水搅匀备用
C₃	12.50 g Na₂SO₃，6.25 g L-半胱氨酸盐酸盐l和2.50 g木瓜酶，加5.00 kg水搅匀备用
C₄	6.25 g Na₂SO₃，3.13 g L-半胱氨酸盐酸盐和1.25 g木瓜酶，加5.00 kg水搅匀备用

脱黏液配方	配制方法
A	1.00 kg黄泥搓碎后，加5.00 kg水浸泡6~8 h，搅匀用40目筛绢滤渣备用
B	200.00 g医用滑石粉和25.00 g食盐，加水5.00 kg搅匀备用
C₁	50.00 g Na₂SO₃，25.00 g L-半胱氨酸盐酸盐和10.00 g木瓜酶，加5.00 kg水搅匀备用
C₂	25.00 g Na₂SO₃，12.50 g L-半胱氨酸盐酸盐和5.00 g木瓜酶，加5.00 kg水搅匀备用
C₃	12.50 g Na₂SO₃，6.25 g L-半胱氨酸盐酸盐l和2.50 g木瓜酶，加5.00 kg水搅匀备用
C₄	6.25 g Na₂SO₃，3.13 g L-半胱氨酸盐酸盐和1.25 g木瓜酶，加5.00 kg水搅匀备用

组别		总卵数/颗	受精卵数/颗	出膜仔鱼数/尾
A		50	41±1	13±2^f
B		50	42±1	25±4^e
C₁	C₁-15 s	50	42±3	8±2^gh
	C₁-30 s	50	42±1	5±2^hi
	C₁-45 s	50	41±3	4±1^ij
	C₁-60 s	50	42±2	2±1^j
C₂	C₂-15 s	50	43±2	33±3^bcd
	C₂-30 s	50	42±3	13±2^f
	C₂-45 s	50	41±2	9±2^g
	C₂-60 s	50	42±2	4±2^ij
C₃	C₃-15 s	50	44±1	42±2^a
	C₃-30 s	50	42±2	39±2^a
	C₃-45 s	50	44±2	36±3^b
	C₃-60 s	50	43±2	31±1^d
C₄	C₄-15 s	50	44±2	26±3^e
	C₄-30 s	50	44±1	26±2^e
	C₄-45 s	50	43±3	32±2^cd
	C₄-60 s	50	41±2	33±2^bc

组别		总卵数/颗	受精卵数/颗	出膜仔鱼数/尾
A		50	41±1	13±2^f
B		50	42±1	25±4^e
C₁	C₁-15 s	50	42±3	8±2^gh
	C₁-30 s	50	42±1	5±2^hi
	C₁-45 s	50	41±3	4±1^ij
	C₁-60 s	50	42±2	2±1^j
C₂	C₂-15 s	50	43±2	33±3^bcd
	C₂-30 s	50	42±3	13±2^f
	C₂-45 s	50	41±2	9±2^g
	C₂-60 s	50	42±2	4±2^ij
C₃	C₃-15 s	50	44±1	42±2^a
	C₃-30 s	50	42±2	39±2^a
	C₃-45 s	50	44±2	36±3^b
	C₃-60 s	50	43±2	31±1^d
C₄	C₄-15 s	50	44±2	26±3^e
	C₄-30 s	50	44±1	26±2^e
	C₄-45 s	50	43±3	32±2^cd
	C₄-60 s	50	41±2	33±2^bc