Abstract:

The aim was to provide a theoretical basis for studying sugar beet U-box E3 ubiquitin ligases from the perspective of bioinformatics. The beet T710-Mu strain was used as the experimental material. The BvPUB gene (plant U-box gene) that was differentially expressed under salt stress was obtained from transcriptome data at the early stage, and bioinformatics methods was used to analyze these PUB genes. They mainly included physical and chemical properties analysis, chromosome localization analysis, gene sequence analysis, domain analysis, family classification analysis and interaction protein prediction. The results showed that the BvPUB genes was localized on all 9 chromosomes, and contained multiple conserved domains of PUB protein, such as U-box, Pkinase, Pkinase-Tyr, Usp, ARM and KAP domains, which could be divided into ClassⅡ, ClassⅢ, ClassⅣ according to structural characteristics of the Arabidopsis U-box family. Sugar beet PUB proteins could act as E3 ubiquitin ligases to interact with a variety of functional proteins to achieve ubiquitination modification. Plant PUB protein had E3 ubiquitin ligase activity, which played an important role in plant growth and development and resistance to environmental stress.

Key words: sugar beet, salt stress, U-box, ubiquitin ligase, bioinformatics