Interaction Analysis of Transcription Factors and Promoters and Its Application in Response of Plants to Stress

doi:10.11924/j.issn.1000-6850.casb2021-0563

Abstract

Abstract:

Transcription factors are important regulatory proteins that regulate gene expression. Transcription factors and the related cis-acting elements of promoters which bind to them both play the role of molecular switches in gene expression, therefore it is particularly important to explore the interaction of transcription factors with promoters. In order to study the regulation mechanism of transcription factors on downstream target genes when plants are subjected to stress, this paper summarized the main transcription factor families involved in stress, the identification of transcriptional activation activity of transcription factors, the interaction analysis technologies between transcription factors and promoters and their applications in plants’ response to stress, which could provide references for the comprehensive and in-depth study of the gene expression regulation mechanism of plants in response to stress.

Key words: transcription factor, promoter, ChIP, DNase I footprinting, EMSA, Y1H, stresses

CLC Number:

S184

Wang Xue, Wang Shenghao, Yu Bing. Interaction Analysis of Transcription Factors and Promoters and Its Application in Response of Plants to Stress[J]. Chinese Agricultural Science Bulletin, 2021, 37(33): 112-119.

Figures/Tables 4

References 70

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逆境类型	物种	转录因子	转录因子家族	靶基因	作用元件	参考文献
青枯雷尔氏菌	辣椒 Capsicum annuum L.	CaWRKY40	WRKY	CaC3H14	W-box	[55]
干旱	水稻 Oryza sativa L.	OsSNAC1	NAC	OsSNAC1TGDs	NACRS、ABRE	[56]
葡萄座腔菌	苹果 Malus domestica (Suckow) Borkh.	MdWRKY15	WRKY	MdICS1	W-box	[57]
高温	核桃 Juglans regia L.	JrGRAS2	GRAS	JrDof3	DOFCOREZM	[58]

逆境类型	物种	转录因子	转录因子家族	靶基因	作用元件	参考文献
青枯雷尔氏菌	辣椒 Capsicum annuum L.	CaWRKY40	WRKY	CaC3H14	W-box	[55]
干旱	水稻 Oryza sativa L.	OsSNAC1	NAC	OsSNAC1TGDs	NACRS、ABRE	[56]
葡萄座腔菌	苹果 Malus domestica (Suckow) Borkh.	MdWRKY15	WRKY	MdICS1	W-box	[57]
高温	核桃 Juglans regia L.	JrGRAS2	GRAS	JrDof3	DOFCOREZM	[58]

逆境类型	物种	转录因子	转录因子家族	靶基因	作用元件	参考文献
大丽轮枝菌	棉花 Gossypium hirsutum L.	GhNAC100	NAC	GhPR3	CGTA-box	[67]
霜霉病菌	大豆 Glycine max (L.) Merr.	GmWRKY31	WRKY	GmSAGT1	W-box	[68]
冷胁迫	虎百合 Lilium lancifolium L.	LlMYB3	MYB	LlCHS2	–	[69]
盐胁迫	水稻 Oryza sativa L.	OsPCF2、OsCPP5、OsNIN-like2~4	TCP、CPP、NIN-like	OsNHX1	–	[70]

逆境类型	物种	转录因子	转录因子家族	靶基因	作用元件	参考文献
大丽轮枝菌	棉花 Gossypium hirsutum L.	GhNAC100	NAC	GhPR3	CGTA-box	[67]
霜霉病菌	大豆 Glycine max (L.) Merr.	GmWRKY31	WRKY	GmSAGT1	W-box	[68]
冷胁迫	虎百合 Lilium lancifolium L.	LlMYB3	MYB	LlCHS2	–	[69]
盐胁迫	水稻 Oryza sativa L.	OsPCF2、OsCPP5、OsNIN-like2~4	TCP、CPP、NIN-like	OsNHX1	–	[70]