CRISPER/Cas9 System in Plant Genome Modification and Crop Genetics and Breeding: Research Progress

doi:10.11924/j.issn.1000-6850.casb2021-0757

Abstract

Abstract:

The CRISPR/Cas9 system is a simple and efficient targeted gene editing technology, which has important application value in plant genetic improvement and crop genetics and breeding. This research mainly introduced the principle and construction method of CRISPR/Cas9, discussed the application and research progress of CRISPR/Cas9 in plant gene function and gene expression regulation, plant genome site-specific editing and crop molecular breeding in recent years. We analyzed the main influencing factors and the optimizing methods of this gene editing technology, as well as problems in its application and the solutions to the problems, and discussed the future development direction of the technology, thus providing reference for the application of CRISPR/Cas9 in plant genome editing and crop genetics and breeding.

Key words: CRISPER/Cas9, gene editing, influencing factors of CRISPR/Cas9 system, molecular design breeding, crop genetics and breeding

CLC Number:

QUAN Ying, ZHANG Xiaojuan, ZHAO Hui, SUN Xiaomin, MA Xiuqi. CRISPER/Cas9 System in Plant Genome Modification and Crop Genetics and Breeding: Research Progress[J]. Chinese Agricultural Science Bulletin, 2022, 38(26): 9-14.

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