Photosynthetic Response of Sugar Beet Seedlings Under the Intervention of Colpoda reniformis

doi:10.11924/j.issn.1000-6850.casb2021-1141

Abstract

Abstract:

In order to investigate the intervention of Colpoda reniformis, a new protozoan species, on the photosynthetic characteristics and growth of sugar beet seedlings, we used liquid culture method, took the breeding strain 701 of Sugar Beet Research Institute of Chinese Academy of Agricultural Sciences as the test material, and set up two treatments of inoculated and uninoculated with Colpoda reniformis. Then, we evaluated the basic phenotypic characteristics and photosynthetic characteristics of sugar beet seedlings under Colpoda reniformis intervention by physiological indicators analysis and correlation analysis. Compared with those of the uninoculated treatment of Colpoda reniformis, the phenotypic characteristics and biomass accumulation of sugar beet seedlings under inoculated treatment were significantly promoted by Colpoda reniformis, and the total plant height, leaf area, root length, dry weight of the aboveground and underground parts increased by 14.14%, 15.49%, 20.09%, 10.0% and 33.3%, respectively. In addition, the contents of Pn, Gs, chlorophyll A, chlorophyll B and total chlorophyll in sugar beet leaves significantly increased by 43.91%, 40.56%, 31.58%, 31.82% and 31.72%, respectively, compared with those of the control. The activity of glutamine synthase (GS) significantly increased by 23.52%. Nitrogen dry matter production efficiency and nitrogen accumulation increased by 6.3% and 8.8% in the aboveground parts and 5.3% and 19.7% in the underground parts, respectively. Correlation analysis showed that photosynthetic characteristic indicators, nitrogen efficiency and plant leaf area played an important role in the growth of sugar beet. Therefore, Colpoda reniformis can interfere with the growth and development of sugar beet seedlings, improve photosynthesis and nitrogen efficiency by increasing leaf area and chlorophyll content, and ultimately promote the growth and biomass accumulation of the seedlings.

Key words: Colpoda reniformis, sugar beet, photosynthetic characteristics, morphological characteristics, physiological indicators

CUI Jingjing, WANG Li, DOU Qianyao, HAN Zhuojun, PAN Hengyan, SONG Baiquan, ZHOU Jianchao, WANG Qiuhong. Photosynthetic Response of Sugar Beet Seedlings Under the Intervention of Colpoda reniformis[J]. Chinese Agricultural Science Bulletin, 2022, 38(36): 23-33.

Figures/Tables 10

References 54

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处理	真叶数	地上部高/cm	总植株高/cm	叶片面积/cm²	根长/cm	叶片长宽比/%	叶长叶柄比/%
CK	7.23±0.77	11.95±1.22	34.30±6.60	19.63±4.25	22.35±6.80	2.19±0.25	1.02±0.20
1W	7.50±0.88^ns	12.31±0.99^ns	39.15±7.77^**	22.67±2.97^***	26.84±7.74^**	2.08±0.19^*	1.10±0.26^ns

处理	真叶数	地上部高/cm	总植株高/cm	叶片面积/cm²	根长/cm	叶片长宽比/%	叶长叶柄比/%
CK	7.23±0.77	11.95±1.22	34.30±6.60	19.63±4.25	22.35±6.80	2.19±0.25	1.02±0.20
1W	7.50±0.88^ns	12.31±0.99^ns	39.15±7.77^**	22.67±2.97^***	26.84±7.74^**	2.08±0.19^*	1.10±0.26^ns

	Pn/[μmol/(m²·s)]	Tr/[mmol/(m²·s)]	Gs/[mmol/(m²·s)]	Ci/[mmol/(m²·s)]
CK	13.05±2.47	2.20±0.34	39.89±8.85	118.47±36.19
1W	18.78±2.68***	2.49±0.75^ns	59.44±13.39**	166.52±68.02^ns

	Pn/[μmol/(m²·s)]	Tr/[mmol/(m²·s)]	Gs/[mmol/(m²·s)]	Ci/[mmol/(m²·s)]
CK	13.05±2.47	2.20±0.34	39.89±8.85	118.47±36.19
1W	18.78±2.68***	2.49±0.75^ns	59.44±13.39**	166.52±68.02^ns

	叶绿素a	叶绿素b	总浓度	地上高	根长	总高	叶长	叶宽	叶面积	地上干质量	地下干质量
叶绿素a	1
叶绿素b	.913^**	1
总浓度	.986^**	.968^**	1
地上部高	-0.018	0.250	0.092	1
根长	-0.143	-0.159	-0.153	0.066	1
总植株高	-0.143	-0.113	-0.133	0.233	.986^**	1
叶长	-0.288	-0.117	-0.224	0.597	-0.060	0.042	1
叶宽	0.298	0.266	0.292	-0.222	0.471	0.421	-0.578	1
叶片面积	-0.098	0.088	-0.024	0.546	0.358	0.442	.702^*	0.174	1
地上干质量	0.201	0.245	0.224	0.192	0.528	0.547	-0.451	.719^*	0.100	1
地下干质量	-0.126	-0.102	-0.119	0.486	-0.128	-0.043	.845^**	-0.389	.666^*	-0.452	1
总生物量	0.167	0.219	0.192	0.531	0.494	0.572	0.078	0.530	0.565	.788^**	0.192
根冠比	-0.171	-0.147	-0.165	0.284	-0.379	-0.322	.818^**	-0.617	0.433	-.782^**	.902^**
氮效率	-0.074	-0.016	-0.052	0.389	-0.369	-0.294	0.296	-0.375	0.026	-0.258	0.374
SPAD	0.154	0.208	0.180	0.337	-0.202	-0.139	-0.248	0.172	-0.138	0.235	-0.092
Pn	-0.121	0.034	-0.060	.797^**	0.116	0.248	0.517	-0.134	0.518	0.393	0.436
Tr	0.421	0.441	0.438	0.310	-0.119	-0.064	-0.178	0.340	0.090	0.565	-0.088
Gs1	0.007	0.166	0.073	0.605	-0.160	-0.054	0.396	-0.239	0.275	0.262	0.234
Ci	-0.063	0.085	-0.003	0.323	-0.327	-0.264	0.323	-0.379	0.060	-0.068	0.108
NR	0.009	0.153	0.068	0.600	-0.062	0.041	0.168	-0.151	0.083	-0.135	0.286
GS	-0.011	-0.155	-0.070	-0.605	0.058	-0.046	-0.174	0.146	-0.093	0.132	-0.293
	总生物量	根冠比	氮效率	SPAD	Pn	Tr	Gs1	Ci	NR	GS
叶绿素a
叶绿素b
总浓度
地上部高
根长
总植株高
叶长
叶宽
叶片面积
地上干质量
地下干质量
总生物量	1
根冠比	-0.238	1
氮效率	-0.047	0.359	1
SPAD	0.185	-0.166	0.617	1
Pn	.719^*	0.131	0.409	0.227	1
Tr	0.578	-0.271	-0.191	0.387	0.384	1
Gs1	0.434	0.056	0.601	0.309	.865^**	0.281	1
Ci	-0.021	0.112	.755^*	0.316	0.524	-0.091	.857^**	1
NR	0.032	0.265	0.491	0.576	0.131	-0.077	-0.007	-0.010	1
GS	-0.040	-0.268	-0.493	-0.577	-0.137	0.073	0.003	0.009	-1.000^**	1