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Chinese Agricultural Science Bulletin ›› 2023, Vol. 39 ›› Issue (3): 49-54.doi: 10.11924/j.issn.1000-6850.casb2022-0357

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The Influencing Factors and Preservation Methods of Plant Pollen: Research Progress

WANG Hui1,2(), ZHANG Conghe1(), HUANG Yanling1, WANG Heting1, FANG Yu1,2, YANG Wei1, GUAN Changhong1, YANG Li1, WANG Lin1, YAN Zhi1   

  1. 1 Anhui Win-all Hi-tech Seed Co., LTD./National Key Laboratory for New Variety Development of Hybrid Rice of Ministry of Agriculture and Rural Affairs, Hefei 230088
    2 Shanghai ZKW Molecular Breeding Technology Co., Ltd., Shanghai 200233
  • Received:2022-05-07 Revised:2022-08-15 Online:2023-01-25 Published:2023-02-01

Abstract:

Pollen is one of the main forms of plant germplasm and it contains all genotypes of the species. Research on the preservation of plant pollen is of great significance for improving the conservation method of germplasm resources and accelerating the breeding process. In order to meet the needs of germplasm resources conservation and hybrid breeding, the authors expounded the effects of pollen types, genotypes, chemical components and environmental factors on plant pollen viability, and summarized main methods of plant pollen collection and viability measurement as well as the main preservation methods of plant pollen such as organic solvent, low pressure, low and ultra-low temperature preservation. It is proposed that ultra-low temperature preservation technology will become an important direction of pollen preservation and research. Further research on the pollen preservation mechanism is necessary. This paper will provide certain reference for studying long-term and high-efficiency pollen preservation methods of different plants.

Key words: pollen, influencing factors, viability, preservation method, ultra-low temperature