Molecular Evolution and Functional Analysis of Histone H2 from Pinctada fucata martensii

doi:10.11924/j.issn.1000-6850.casb2022-0812

Abstract

Abstract:

The aim of this research was to investigate the molecular evolutionary characteristics of histone H2 and its role in the immune regulation in pearl oyster Pinctada fucata martensii, and to provide a theoretical basis for further elucidation of the physiological functions of histone H2 in Pinctada fucata martensii. Bioinformatics methods were used to identify histone H2A and H2B from nine species, such as Pinctada fucata martensii, Homo sapiens, and Crassostrea gigas, and their molecular evolution was analyzed. Using the data available in our previous research, the functions of histone H2A and H2B in the development, tissue and immunity of Pinctada fucata martensii were also analyzed. The main results showed that the human genome contained 27 histone H2A and 20 histone H2B, the C. gigas genome contained 12 histone H2A and 8 histone H2B, and the genome of Pinctada fucata martensii contained only two H2A (H2A-1 and H2A-2) and one H2B, which showed a contraction phenomenon compared with other species. Sequence analysis revealed that there were 6 H2A and 2 H2B variants in human genome, 3 H2A and 1 H2B variants in C. gigas, and 1 H2A variant (H2A-2) in Pinctada fucata martensii. The expression of H2A-1 and H2B were changed after transplantation in Pinctada fucata martensii, indicating that they were involved in the immunological regulation of the transplantation immune response. The expression level of H2A-1 gene was higher in the gastrula stage, H2A-2 was highly expressed in trochophore stage and type D larvae, and the expression level of H2B was higher in the egg and fertilized egg. H2A-1 was relatively high in pearl sac, the expression level of H2A-2 was relatively high in blood cell, foot, and mantle pallium, and H2B gene was relatively high in gonad and pearl sac. The genome of pearl oyster Pinctada fucata martensii contained only two H2 genes, and both of them involved and played a different role in the regulation of transplantation immune response in Pinctada fucata martensii.

Key words: Pinctada fucata martensii, histone H2, mollusk, evolution

ZHANG Ming, YANG Shuai, CAO Yanfei, LU Xiaowen, JIAO Yu. Molecular Evolution and Functional Analysis of Histone H2 from Pinctada fucata martensii[J]. Chinese Agricultural Science Bulletin, 2023, 39(28): 151-157.

Figures/Tables 5

References 38

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