Abstract:

The aims were to explore the main virus types and sequence information of Jiangxi Yanshan red bud taro, and provide a basis for its virus-free plantlet cultivation. LncRNA sequencing technology was used to determine the virus species of Jiangxi Yanshan red bud taro, and RT-PCR technology, bioinformatics analysis software, and qRT-PCR technology were used for virus validation, sequence analysis, and tissue expression analysis. Two viruses, taro mosaic virus (DsMV) and cauliflower mosaic virus (CaMV), were detected in red bud taro from Yanshan, Jiangxi. The total cDNA lengths of DsMV and CaMV genes were 1780 bp and 5412 bp, respectively, encoding 592 and 1803 amino acids. The protein secondary structure of DsMV and CaMV poly protein was composed of alpha helix, extended strand and random coil, and its tertiary structure was composed of monomeric proteins. DsMV and CaMV were both infected in the roots, stems, and leaves of Jiangxi Yanshan red bud taro, but both had the highest expression levels in the leaves. The main types of viruses infecting Jiangxi Yanshan red bud taro had been identified, and CaMV had been detected for the first time in Jiangxi Yanshan red bud taro.

Key words: Jiangxi Yanshan red bud taro, identification of virus species, lncRNA sequencing, DsMV, CaMV, sequence analysis