Chinese Agricultural Science Bulletin ›› 2007, Vol. 23 ›› Issue (5): 71-71.
Special Issue: 小麦
• 生物技术科学 • Previous Articles Next Articles
Cai Dongming, Ji Wanquan, Ren Zhilong, Wang Qiuying, Wang Changyou, Zhang Hong, Wang Yajuan
Online:
Published:
Abstract: Culture medium affects seedling production of wheat haploid. Growth and development of 16 single F1 on eight kinds of medium were studied in order to find a kind of the optimal culture medium for growth of wheat young embryo. Average seedling production frequency of 8 eight kinds of medium were 32.71%, 41.48%, 47.15%, 53.59%, 57.98%, 62.05%, 53.26% and 42.21%, respectively. Average seedling production frequency of culture medium ①, ②, ③ and ⑧ were lower and that of ④, ⑤, ⑥ and ⑦ were higher. Seedling production frequency of the medium ⑥, (1/2MS+0.2 mg/L IAA+0.2 mg/L6-BAP+2% sucrose +7.5g/L agarose, PH=5.8), was 62.05%. The young germnation differentiation of the medium ⑥ was obvious, and roots curved to the bottle bottom and leaf was deeper green. The results showed that the culture effect of the medium ⑥ was better than that of the other.
CLC Number:
S512.1+1
Cai Dongming, Ji Wanquan, Ren Zhilong, Wang Qiuying, Wang Changyou, Zhang Hong, Wang Yajuan. Studies on Culture Medium of Wheat Haploid Breeding[J]. Chinese Agricultural Science Bulletin, 2007, 23(5): 71-71.
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https://www.casb.org.cn/EN/Y2007/V23/I5/71