Abstract: Ginger was bred by tissue culture technique, which showed the medium MS+6-BA2.0mg/L+NAA0.1mg/L is best. The tube-plantlets were tested by hardening and transplanting, which showed the harden plantlets is best to raise transplanting survival rate. The optimal treatment are both stronger plantlets and hardening plantlets. The first generation ginger tuber growing out of the tube-plantlets is smaller and healthy, which is suitable to use as seed tuber. The second generation ginger tuber is larger and higher yield, which has good value to trade and economic benefit。