Abstract: Culture medium affects seedling production of wheat haploid. Growth and development of 16 single F1 on eight kinds of medium were studied in order to find a kind of the optimal culture medium for growth of wheat young embryo. Average seedling production frequency of 8 eight kinds of medium were 32.71%, 41.48%, 47.15%, 53.59%, 57.98%, 62.05%, 53.26% and 42.21%, respectively. Average seedling production frequency of culture medium ①, ②, ③ and ⑧ were lower and that of ④, ⑤, ⑥ and ⑦ were higher. Seedling production frequency of the medium ⑥, (1/2MS+0.2 mg/L IAA+0.2 mg/L6-BAP+2% sucrose +7.5g/L agarose, PH=5.8), was 62.05%. The young germnation differentiation of the medium ⑥ was obvious, and roots curved to the bottle bottom and leaf was deeper green. The results showed that the culture effect of the medium ⑥ was better than that of the other.