Abstract:

Abstract: In order to strengthen the detection on the liver mitochondrial proteins which differentially expressed when infected by aflatoxin B1, to prevent the outbreak and spread of poisoning. the toxin production by Aspergillus flavus were optimized and were extraced to infect mice. After 10 weeks, we extraced the mitochondrial protein of the mice and reseached the effect of the Aflatoxin on the mitochondrial proteome of the mice by two-dimensional electrophoresis. The results of analysis of two-dimensional electrophoresis revealed that there were obviously 31 differential protein spots, among which 7 protein spots were new or upregulated and 6 protein spots were disappeared or downregulated. These spots were mostly in the range of 5.2-9.0pH and 8-165 kDa. We speculated that aflatoxin may effect mitochondrial function by the influence on these proteins, which induce the toxicity on mice.