Abstract:

According to the genomic sequence of PCV-2(porcine circovirus type 2) published in GenBank, which designed a pair of specific primers by using Primier5. The total DNA of Pig spleen extracted by CTAB and DNA Kit. And the PCV-2 gene conserved sequence of Pig is amplificated by PCR. Then we study the optimization of the PCR process, and proceduring on the PCR sensitivity and repeatability. The result showed that amplified fragment was about 573 bp. The best PCR program is 30 cycles of 94°C 30 sec, 60°C 30 sec, 72°C 30 sec. The template DNA dilution was 100 times the minimum, which can detect the quality of the minimum template DNA was 25.8 ng. The repeatability of PCR program is better. The detection method provides a method and basis of the rapid detection of PCV-2.