Abstract:

To obtain high purity Yak Endometrium Stromal Cells, yak endometrium tissue was digested by collagenase, stromal cells was isolationed and cultured in vitro, the growth characteristics and immunocytochemical staining was studied. The results showed that the digest effect was good with 2 g/L collagenase Ⅱ at 37℃ working 2.5 h, following replacing with fresh 2 g/L collagenase Ⅱ working 2.5 h; epithelium cells were able to be wiped off through 74 μm filter, followed by 400 r/min centrifugalization and sedimentation, large numbers of single cells were obtained. Two shapes of cells with the former of spinder and polygonal were found when they became adherent. It was verified that this 2 shapes of cells were positive for vimentin, with 95% of positive rate, and negative for cytokeratin by immunocytochemical staining method. The conclusion can be drawn that purified yak stromal cells were obtained.