Welcome to Chinese Agricultural Science Bulletin,

Chinese Agricultural Science Bulletin ›› 2012, Vol. 28 ›› Issue (24): 270-277.

Special Issue: 园艺

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Study on the Separation and Identification of Polyphenols in Persimmon Using LC/MS Coupling Techniques

  

  • Received:2012-06-28 Revised:2012-07-26 Online:2012-08-25 Published:2012-08-25

Abstract:

Persimmon is rich in natural phenolic compounds which have varieties of physiological functions. To separate and identify the polyphenols in persimmon, HP2MGL macroporous adsorption resin, Sephadex LH-20 polysaccharide gels and ODS-A reverse-phase media were employed orderly. Four different phenols in Diospyros kaki L. cv. Mopan, gallic acid, catechin, (-)-epicatechin and caffeine acid, were obtained, which were detected and identified using LC/MS method. The purity of gallic acid, catechin, (-)-epicatechin and caffeine acid was 95.15%, 95.04%, 87.47% and 85.0% respectively. An effective method of isolating and purifying phenolic components from Diospyros kaki L. cv. Mopan was established in this study.