The Analysis of Genetic Diversity of Microsatellite Loci in ‘Zaosheng Cattle’

doi:10.11924/j.issn.1000-6850.2013-3219

Chinese Agricultural Science Bulletin ›› 2015, Vol. 31 ›› Issue (2): 1-7.doi: 10.11924/j.issn.1000-6850.2013-3219

Special Issue: 生物技术；畜牧兽医

The Analysis of Genetic Diversity of Microsatellite Loci in ‘Zaosheng Cattle’

Wang Xiaojin, Huo Shendong, Chen Shien, Long Ling, Gao Xudong, Ye Yongli, He Xinrui

(College of Life Sciences and Engineering, Northwest University for Nationalities, Lanzhou 730030)

Received:2013-12-09 Revised:2014-12-06 Accepted:2014-12-10 Online:2015-03-19 Published:2015-03-19

Abstract

Abstract: To analyze the genetic diversity of ‘Zaosheng cattle’ in the molecular level, polymorphism distributions of microsatellite loci which including IDVGA46, ILSTS005, IDVGA27, TGLA44, IDVGA2, BM2113 and ETH10 were detected in ‘Zaosheng cattle’ by Polymerase Chain Reaction and Polyacrylamide Gel Electrophoresis. Number of alleles and effective alleles, expected heterozygosity and polymorphic information content were calculated. The results showed that a total of 25 alleles were identified on the 7 microsatellite loci with polymorphism from 112 samples. The average number of alleles and effective alleles was 3.5714 and 3.0004, respectively. The average expected heterozygosity was 0.6996 and the average polymorphic information content was up to 0.6526. The results indicated that genetic heterozygosity of ‘Zaosheng cattle’ was relatively high, and the genetic diversity was rich. The selected microsatellite loci could be used to evaluate genetic diversity of ‘Zaosheng cattle’.

Wang Xiaojin,Huo Shendong,Chen Shien,Long Ling,Gao Xudong,Ye Yongli and He Xinrui. The Analysis of Genetic Diversity of Microsatellite Loci in ‘Zaosheng Cattle’[J]. Chinese Agricultural Science Bulletin, 2015, 31(2): 1-7.

References

[1] 姜西安,张力.早胜牛调查[J].中国牛业科学,2007,33(3):92-94.
[2] 张跟喜,丁馥香,王金玉,等.利用微卫星标记分析边鸡遗传多样性及保种效果[J].农业生物技术学报,2010,18(5):944-950.
[3] 管峰,杨利国,贾名威,等.微卫星的构成及其检测技术[J].生物学杂志,2004,21(2):1-3.
[4] 张自富,昝林森,王志刚,等.14个中外黄牛品种的遗传多样性分析[J].西北农林科技大学学报,2007,35(2):27-31.
[5] Skinner D M, Beattie W G, Blatner F R, et al. The sequence of a hermit crab satellite DNA in (TAGG)n-(TAGG)n[J]. Biochemistry,1974,13:3930-3937.
[6] Rendo F, Iriondo M, Jugo B M, et al. Analysis of the genetic structure of endangered bovine breeds from the western pyrenees using DNA microsatellite markers[J]. Biochemical Genetics,2004,42(3-4):99-108.
[7] Ma Y, Cao H, Chen Y, et al. Study on genetic diversity of some cattle breeds[J]. Agricultural Sciences in China, 2003,2(7):804-808.
[8] 冯冬梅,赵会静,罗玉柱,等.牦牛和黄牛三个微卫星基因座种间特异性等位基因分析[J].甘肃农业大学学报,2010,45(4):22-27.
[9] 胡雄贵,张佰忠,刘莹莹,等.应用微卫星标记分析湘西黄牛与其他黄牛品种的亲缘关系[J].家畜生态学报,2011,32(1):24-27.
[10] 常振华.中国黄牛Y染色体微卫星和SNPs遗传多样性与起源研究[D].陕西:西北农林科技大学,2011:1-56.
[11] 曹阳.肉牛微卫星DNA的群体遗传变异分析及其与肉用性状关系的研究[D].吉林:延边大学,2003:1-77.
[12] 曾庆国,陈艺燕.微卫星位点筛选方法综述[J].生态科学,2005,24(4):368-372.
[13] 阮泓越,宛煜嵩,贺辉群,等.14个微卫星DNA标记在猪个体识别和溯源中的应用研究[J].农业生物技术学报,2010,18(6):1129-1133.
[14] Ciampolini R., Leveziel H, Mazzanti E, et al. Genomic identification of the breed of an individual or its tissue[J]. Meat Science, 2000,54(1):35-40.
[15] 吴伟,王栋,曹红鹤.微卫星DNA标记对5个中外黄牛品种/群体遗传结构的研究[J].吉林农业大学学报,2000,22(4):5-10.
[16] 侯冠玉,马月辉,道尔吉.微卫星DNA在草食动物遗传育种中的研究与应用[J].中国草食动物,2003,23(3):39-40.
[17] 李荣岭,张桂香,王志刚,等.微卫星标记对12个中外牛品种群体遗传结构的研究[J].遗传,2007,29(12):1463-1470.
[18] 王洪程,昝林森,宋付标.秦川牛微卫星基因位点遗传多样性分析[J].中国农学通报,2011,27(11):10-14.
[19] 李铎,柴志欣,姬秋梅,等.西藏牦牛微卫星DNA的遗传多样性[J].遗传,2013,35(2):175-184.

The Analysis of Genetic Diversity of Microsatellite Loci in ‘Zaosheng Cattle’

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 0

Recommended Articles

Metrics

Comments

For authors

For reviewers

Reader center

Contact us