Abstract: The village is a kind of district unit for rural tourism development which is an important way to develop rural economy, promote industrial upgrading and transformation, increase farmers’income, and solve many social problems. Different from other tourist destination types, the nature of rural tourist destinations is the rurality. Based on rurality and rural tourism, the index system was organized by three dimensions of residents, settlement and industry, in which the weight of each indicator was derived from the Analytic Hierarchy Process, and then the rurality comprehensive assessment model was constructed. Chosen as a case, Shiliuhong Village in the suburb of WuHan was graded by 5 experts using indicators and its rurality was assessed. The result showed that settlement rurality was the most obvious characteristics, followed by its industry rurality and resident rurality, and the overall rurality was relatively high. The comprehensive assessment model introduced in this paper, simple and practical, was suitable for horizonal comparison and dynamic monitor on rurality of rural tourist destinations, which could provide supports on decision making for development and management of rural tourist destinations.