Abstract: In order to provide a scientific basis for the study on responses of rice to temperature stress, the effect of temperature on rice yield at different growth stages was investigated. A 7-day temperature control experiment was conducted in artificial intelligence climatic chamber by the method of pot culture, the effect of temperature on rice yield and its components were observed in tillering stage, booting stage and the filling stage. The results showed that the biomass followed the order of low temperature＞ middle temperature＞ high temperature and filling stage＞ booting stage＞ tillering stage; the change of temperature had great effect on productive panicle in tillering stage and booting stage, and had little effect on productive panicle in filling stage; temperature control had the most obvious effect on grain forming in booting stage, and high temperature caused the reduction of grain forming percentage; high temperature reduced thousand seed weight in booting stage, and the difference was not significant in other stages; yield of per plant followed the order of low temperature＞ middle temperature＞ high temperature and filling stage＞ tillering stage＞ booting stage, high temperature treatment had the lowest yield in booting stage, which was reduced by 26.80% to 42.46% and 21.16% to 36.22% in comparing with that of low temperature treatment and middle temperature treatment in the same stage respectively. The results indicated that high temperature had the greatest effect on early rice yield and yield components, and the booting stage was the most sensitive stage.