Abstract: To effectively evaluate the effect of biocontrol bacterium against Fusarium oxysporum f. sp. cubense, which causes banana fusarium wilt, 3 antagonistic strains A5-6, D7-16 and CL7 were selected from 249 testing isolates. The antibacterial ability of the 3 strains was observed, and resistant spectrum tests and acteriostatic ability monitoring in soil and potted seedlings were conducted. Then morphological characteristics of the 3 strains were observed and identification of the 3 strains was conducted using 16S rDNA sequence. Microscopic observation showed that the pathogen hyphae growth and spores germination were inhibited by A5-6, D7-16 and CL7. Resistance spectrum tests showed that the 3 strains had relatively high resistant rate to FOC4 (60%) and relatively low resistant rate to other specializations of Fusarium oxysporum (40%-50%). The population of FOC4 in the soil of potted seedlings was smaller than that in the soil without potted seedlings under the control of the 3 strains. Pot experiment showed that A5-6 had better disease control ability (72.3%) than that of D7-16 (45.4%) and CL7 (50.0%). The morphological characteristics and 16S rDNA sequence analysis showed that the 3 strains were all Bacillus sp., and D7-16 was Brevibacillus brevis. All the 3 strains should be used as bio-control bacterium to control banana fusarium wilt and further developmental research should be conducted.