Genetic Analysis and Gene Fine Mapping of A High-tillering and Dwarf Mutant htd7(t) in Rice (Oryza sativa L.)

doi:10.11924/j.issn.1000-6850.casb16050072

Abstract

Abstract: Tillering is one of the most important agronomical characters of rice that determines the final yield. A rice mutant with a high-tillering capacity and dwarf, designated as htd7(t), was isolated from the offspring of the japonica rice (Oryza sativa L. ssp. japonica) variety‘Nipponbare’treated with 350 Gy 60Co-γ radiation. To clone the HTD7(t) locus, positive and negative cross combinations were developed between htd7(t) and‘9311’, genetic analysis found that htd7(t) was controlled by one recessive nuclear gene. HTD7(t) was roughly mapped between two microsatellite markers RM21 and RM254 on the chromosome 11, with genetic distances of 5.6 cM and 3.2 cM, respectively. According to the published rice genome data, 13 new InDel (Insertion-Deletion) markers were developed around HTD7(t), and then fine mapping of HTD7(t) was conducted. The BAC contig of HTD7(t) was constructed based on the mapping results, and HTD7(t) was further mapped between two InDel markers, InDel11-3 and InDel11-5, with physical distance of 64.8 kb.

CLC Number:

S330

References

[1] 王永胜,王景,段静雅,等. 水稻极度分蘖突变体的分离和遗传学初步研究[J]. 作物学报,2002,28(2)：235-239.
[2] 赵芳明, 刘桂富, 朱海涛, 等. 用单片段代换系对不同时期水稻分蘖数QTL的非条件和条件定位[J]. 中国农业科学,2008,41(2)：322-330.
[3] 李万昌, 王俊伟, 余娇娇.水稻分蘖基因的研究概况[J]. 作物杂志,2012, 3：19-22.
[4] Xu Y B, Shen Z T. Diallel analysis of tiller number at different growth stages in rice(Oryza sative L.) [J]. Theoretical and Applied Genetics, 1991, 83(2): 243-249．
[5] Yan J Q,Zhu J,He C X,et al. Quantitative trait loci analysis for the developmental behavior of tiller number in rice (Oryza sativa L.) [J]. Theoretical and Applied Genetics, 1998, 97(1): 267-274.
[6] Wu W R, Li W M, Tang D Z, et al．Time-related mapping of quantitative trait loci underlying tillering number in rice[J]. Genetics, 1999, 151(1) : 297-303.
[7] 任翔,翁清妹,祝莉莉,等．水稻分蘖能力QTL 的定位[J]．武汉大学学报：理学版,2003,49(4)：533-537.
[7] Fujita D, Ebron L A, Araki E, et al.SSFine mapping of a gene for low-tiller number,SLtn, in japonica rice (Oryza sativaSL.) variety Aikawa[J].SSTheoretical and Applied Genetics,S2010,S120(6): 1233-1240.
[8] Ariyaratne M, Takamure I, Kato K, et al.SSShoot branching control by reduced culm number 4 in rice (Oryza sativaSL.)[J].SSSPlant Science,S2009,S176(6): 744-748.
[9] Zhang B S, Tian F, Tan L B,SSet al. Characterization of a novelShigh-tillering dwarf 3Smutant in rice[J].SJournal of genetics and genomics,S2011,S38(9): 411-418.SS
[10] 高振宇,刘晓辉,郭龙彪,等.SS一新的水稻多蘖矮秆突变体tddl(t)的分离及基因的精细定位[J].SS科学通报,2009,54(9)： 1238-1243.
[11] 徐建龙,李春寿,王俊敏,等. 空间环境诱发水稻多蘖矮秆突变体的筛选与鉴定[J].SS核农学报,2003,17(2)：90-94.
[12] Li X Y, Qian Q, Fu Z M,SSet al.SSControl of tillering in rice[J].SNature, 2003, 422(6932): 618-621.
[13] Koumoto T, Shimada H, Kusano H,SSet al. Rice monoculm mutationSmoc2, which inhibits outgrowth of the second tillers, is ascribed to lack of a fructose-1,6-bisphosphatase[J]. Plant Biotechnology, 2013, 30(1): 47-56.
[15] Lu Z F, Shao G N, Xiong J S,SSet al. MONOCULM 3, an Ortholog ofSWUSCHELSin Rice, Is Required for Tiller Bud Formation[J]. Journal of genetics and genomics,S2015,S42(2): 71-78.
[16] Ishikawa S, Maekawa M, Arite T, et al. Suppression of tiller bud activity in tillering dwarf mutants of rice[J]. Plant and Cell Physiology,S2005,S46(1): 79-86.
[17] Arite T, Iwata H, Ohshima K,SSet al.SSDWARF10, anSRMS1/MAX4/DAD1Sortholog, controls lateral bud outgrowth in rice[J].SSThe Plant Journal,S2007,S51(6): 1019-1029.
[18] Lin H, Wang R X, Qian Q,Set al. DWARF27, an iron-containing protein required for the biosynthesis of strigolactones, regulates rice tiller bud outgrowth[J]. The Plant Cell,S2009,S21(5): 1512-1525.
[19] Jiang L, Liu X, Xiong G S, et al.SDWARF 53 acts as a repressor of strigolactone signalling in rice[J].SNature,S2013,S504(7480): 401-405.
[20] Tong H N, Jin Y, Liu W B, et al.SDWARF AND LOW-TILLERING, a new member of the GRAS family, plays positive roles in brassinosteroid signaling in rice[J].SThe Plant Journal,S2009,S58(5): 803-816.
[21] Liu SK, Liu L L, Ren Y L, et al. Dwarf and tiller-enhancing 1Sregulates growth and development by influencing boron uptake in boron limited conditions in rice[J]. Plant Science,S2015,S236: 18-28.
[22] Zou J H, Zhang S Y, Zhang W P, et al.SThe riceSHIGH-TILLERING DWARF1Sencoding an ortholog of Arabidopsis MAX3 is required for negative regulation of the outgrowth of axillary buds[J].SThe Plant Journal,S2006,S48(5): 687-698.
[23]SLiu W Z, Wu C, Fu Y P, et al.SSIdentification and characterization ofSHTD2: a novel gene negatively regulating tiller bud outgrowth in rice[J]. Planta,S2009,S230(4): 649-658.
[24] Takeda ST, Suwa Y, Suzuki M, et al.STheSOsTB1Sgene negatively regulates lateral branching in rice[J].SSThe Plant Journal,S2003,S33(3): 513-520.
[25] Wang W F, Li G, Zhao J, et al.SDWARF TILLER1, a WUSCHEL-Related Homeobox Transcription Factor, Is Required for Tiller Growth in Rice[J].SSPLoS Genetics,S2014,S10(3): e1004154.
[26] Dellaporta S L, Wood J, Hicks J B. A plant DNA minipreparation: Version II [J]. Plant Mol Biol Rep, 1983, 1(4): 19-21.
[27] Shen Y J, Jiang H, Jin J P, et al. Development of genome-wide DNA Polymorphism database for map-based cloning of rice genes[J]. Plant Physiol, 2004, 135(3): 1198-1205.
[28] Michelmore R W, Paran I, Kesseli R V. Identification of markers linked to disease-resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions by using segregating populations[J]. Proc Natl Acad Sci USA, 1991, 88(21): 9828-9832.
[29] Lander E S, Green P, Abrahamson J, et al. Mapmaker: An interactive computer package for constructing primary genetic linkage maps of experimental and natural populations[J]. Genomics, 1987, 1(2): 174-181.

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