Abstract: In order to verify the scientific application of stabilized nitrogen fertilizer in rape, a field experiment was carried out in Anhui Province during 2014 and 2015 to investigate the effects of stabilized N fertilizer (SN) rates and its application methods on rice yield, nitrogen accumulation, nitrogen use efficiency (NUE) of rape, economic efficiency and soil nutrients. Compared to non-stabilized nitrogen fertilizer, the yield of SN treatments (as 100% N one application, 100% N split application, 85% N one application and 70% N one application) increased 225.6%, 232.5%, 206.8% and 181.2% respectively. Total N accumulated by above ground parts increased 309.2%, 327.6%, 254.0% and 202.1%, and economic benefits increased 287.9%, 273.9%, 266.1% and 234.0% respectively. Compared to local farmer fertilizer practice, the yield of SN treatments (as 100% one application and 100% split application) increased 8.2% and 10.5% respectively. Total N absorbed by above ground parts increased 15.2% and 20.4%, and economic benefits increased 16.8% and 12.6% respectively. At the same N rate, SN with 100% promoted significantly N accumulation than farmer fertilizer practice. The nitrogen apparent efficiency of SN was significantly higher than farmer fertilizer practice, 100% SN had even more significant difference than 85% and 70% SN, and 100% SN with the split application was the highest. The agronomic efficiency and partial productivity of SN was higher than farmer fertilizer practice. Based on rape yield and NUE, the optimum SN application rate was 180 N kg/hm2 with the ratio of basal fertilization to spring fertilization of 6:4.