Different Exogenous Hormones in the Process of Callus Induction: Effects on the Browning Rate of Oil Palm Leaf

doi:10.11924/j.issn.1000-6850.casb18070015

Abstract

Abstract: The paper aims to study the influence of different plant hormones on the browning rate of explants during the tissue culture process of mature oil palm leaves. Taking the mature oil palm leaves as the explants, we treated them with different hormones as 2,4-D, NAA, IBA, IAA, KT, TDZ and 6-BA, to analyze the browning rate of oil palm leaves under induction. The results showed that: (1) the 6-BA and IBA had the highest browning rate for mature leaves than other kinds of plant hormones, and the browning rate was 66.98% and 65.35%, respectively; (2) the browning rate of mature leaves treated with KT was significantly lower than that treated with 6-BA, IBA and TDZ; (3) the browning rate of mature leaves treated with 2,4-D, NAA, IAA had no significant differences. It is suggested that KT should be given priority under the condition of similar callus induction rate.

CLC Number:

References

[1] Abdullah R, Wahid MB. World Palm Oil Supply, Demand, Price and Prospects: Focus on Malaysian and Indonesian Palm Oil Industry[M]. Malaysia: Malaysian Palm Oil Board Press, 2010:1-30.
[2] Kiple KF, Ornelas KC. The Cambridge World History of Food. Vol. 1. [M]. New York: Cambridge University Press; 1999: 397-411.
[3] Low ET, Alias H, Boon SH, et al. Oil palm (Elaeis guineensis Jacq.) tissue culture ESTs: identifying genes associated with callogenesis and embryogenesis[J]. BMC Plant Biol, 2008, 8:1-19.
[4] 曾宪海, 潘登浪, 邹积鑫, 等. 油棕组织培养[J]. 中国热带农业, 2012, 5:35-42.
[5] 姚行成, 邹积鑫, 曾宪海, 等. 油棕组织培养研究进展[J]. 热带作物学报, 2012, 33(3):589-594.
[6] Wang Y, Li K, Song X, et al. Characterization and Comparative Expression Profiling of Browning Response in Medinilla formosana after Cutting[J]. Front Plant Sci, 2016, 7:1897.
[7] Xu C, Zeng B, Huang J, et al. Genome-wide transcriptome and expression profile analysis of Phalaenopsis during explant browning.[J] PLoS One, 2015, 10(4):1-24.
[8] Guan Y, Li SG, Fan XF, et al. Application of Somatic Embryogenesis in Woody Plants[J]. Front Plant Sc, 2016, 7:938.
[9] Liu CP, Yang L, Shen HL. Proteomic Analysis of Immature Fraxinus mandshurica Cotyledon Tissues during Somatic Embryogenesis: Effects of Explant Browning on Somatic Embryogenesis[J]. Int J Mol Sci 2015, 16(6):13692-13713.
[10] Rana MM, Han ZX, Song DP, et al. Effect of Medium Supplements on Agrobacterium rhizogenes Mediated Hairy Root Induction from the Callus Tissues of Camellia sinensis var. sinensis[J]. Int J Mol Sci, 2016, 17(7).
[11] Jones AM, Saxena PK. Inhibition of phenylpropanoid biosynthesis in Artemisia annua L.: a novel approach to reduce oxidative browning in plant tissue culture[J]. PLoS One, 2013, 8(10):1-13.
[12] Dong YS, Fu CH, Su P, et al. Mechanisms and effective control of physiological browning phenomena in plant cell cultures[J]. Physiol Plant, 2016, 156(1):13-28.
[13] 周宜君, 周生闯, 刘玉, 等. 植物生长调节剂对植物愈伤组织的诱导与分化的影响[J]. 中央民族大学学报：自然科学版, 2007, 16:23-28.
[14] 徐晓峰, 黄学林. TDZ:一种有效的植物生长调节剂[J]. 植物学报, 2003, 20:227-237.
[15] 崔元方, 龚峥. 油棕组织培养[J]. 热带农业科学, 1986:70-77.
[16] Ahee J, 林辉奉. 通过体细胞发生胚进行油棕的体外无性繁殖[J]. 世界热带农业信息, 1982:43-44.
[17] 藤田匡, 方伟: 油棕(Elaeis guineensis Jacq)的组织培养[J]. 广西林业科学, 1987:43-48.
[18] 龚峥, 崔元方, 陈幸华, 等, 油棕叶片愈伤组织的诱导[J]. 热带农业科学, 1987:15-18.
[19] 邹积鑫, 尤丽莉, 林位夫. 影响油棕叶片愈伤组织诱导因素研究[J]. 热带农业科学, 2014:54-58.
[20] 沈雁, 王业桐, 曹红星, 等. 油棕的组织培养及其生理生化研究[J]. 江西农业学报, 2012, 24:41-42.
[21] 李娟, 彭金灵, 康娟, 等油棕高效胚培养技术研究[J]. 热带农业科学, 2012, 32:5-8.
[22] Singh R, Ong-Abdullah M, Low ET, et al. Oil palm genome sequence reveals divergence of interfertile species in Old and New worlds[J]. Nature, 2013, 500(7462):335-339.
[23] Singh R, Low ET, Ooi LC, et al. The oil palm SHELL gene controls oil yield and encodes a homologue of SEEDSTICK[J]. Nature, 2013, 500(7462):340-344.
[24] Ong-Abdullah M, Ordway JM, Jiang N, et al. Loss of Karma transposon methylation underlies the mantled somaclonal variant of oil palm[J]. Nature, 2015, 525(7570):533-537.