BvM14-STPK Protein Kinase Interaction Protein in Beta vulgaris: Identification and Screening

doi:10.11924/j.issn.1000-6850.casb20190600323

Abstract

Abstract:

To further explore the signal pathway of BvM14-STPK protein kinase in response to salt stress in sugar beet, the interaction proteins of BvM14-STPK protein kinase were identified and screened in this study. Transgenic tobacco was obtained by transgenic technology, and the protein complex was identified by affinity purification tandem mass spectrometry (TAP/MS). The candidate interaction proteins of BvM14-STPK protein kinase were obtained by searching the tobacco protein database, and the RNA-sequencing data of sugar beet M14 was used to analyze the transcription level of the genes which encoded the candidate interaction proteins under salt stress. The results showed that, three BvM14-STPK protein candidate interaction proteins were obtained under non-salt treatment, and six candidate interaction proteins were obtained under salt treatment in the tobacco database. The transcriptome data analysis under salt stress showed that four interaction protein-coding genes responded to salt stress at different tissues and different salt concentrations. It is a positive foundation for further verification of the interaction between BvM14-STPK protein kinase and its candidate interaction proteins in plants and the salt resistance mechanism of sugar beet.

Key words: sugar beet M14, protein kinase, TAP/MS, protein-protein interaction, salt stress

CLC Number:

S566.3

Lv Jiabao, Yu Mengdi, Li Haiying. BvM14-STPK Protein Kinase Interaction Protein in Beta vulgaris: Identification and Screening[J]. Chinese Agricultural Science Bulletin, 2020, 36(1): 30-34.

Figures/Tables 4

References 21

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Accession		Description	Coverage	#Peptides	# Unique Peptides	#AAs	MW/kDa	pI
XP_009771888.1	AB	PREDICTED: heat shock 70 kDa protein-like [Nicotiana sylvestris]	3.490136571	2	2	659	72.292	5.39
XP_016439103.1	AB	PREDICTED: DNA repair protein RAD16-like [Nicotiana tabacum]	1.187904968	2	2	926	104.76	8.22
XP_016437499.1	AB	PREDICTED: chaperone protein ClpB1 [Nicotiana tabacum]	3.951701427	4	4	911	101.35	6.21
XP_016475992.1	B	PREDICTED: probable serine/ threonine-protein kinase At1g54610 [Nicotiana tabacum]	2.456140351	2	2	570	65.306	9.61
XP_009775590.1	B	PREDICTED: zinc finger BED domain-containing protein RICESLEEPER 1-like, partial [Nicotiana sylvestris]	1.431980907	2	2	838	94.175	7.93
XP_016495333.1	B	PREDICTED: L-type lectin-domain containing receptor kinase S.4-like [Nicotiana tabacum]	2.026049204	2	2	691	77.049	6.68

Accession		Description	Coverage	#Peptides	# Unique Peptides	#AAs	MW/kDa	pI
XP_009771888.1	AB	PREDICTED: heat shock 70 kDa protein-like [Nicotiana sylvestris]	3.490136571	2	2	659	72.292	5.39
XP_016439103.1	AB	PREDICTED: DNA repair protein RAD16-like [Nicotiana tabacum]	1.187904968	2	2	926	104.76	8.22
XP_016437499.1	AB	PREDICTED: chaperone protein ClpB1 [Nicotiana tabacum]	3.951701427	4	4	911	101.35	6.21
XP_016475992.1	B	PREDICTED: probable serine/ threonine-protein kinase At1g54610 [Nicotiana tabacum]	2.456140351	2	2	570	65.306	9.61
XP_009775590.1	B	PREDICTED: zinc finger BED domain-containing protein RICESLEEPER 1-like, partial [Nicotiana sylvestris]	1.431980907	2	2	838	94.175	7.93
XP_016495333.1	B	PREDICTED: L-type lectin-domain containing receptor kinase S.4-like [Nicotiana tabacum]	2.026049204	2	2	691	77.049	6.68