Welcome to Chinese Agricultural Science Bulletin,

Chinese Agricultural Science Bulletin ›› 2020, Vol. 36 ›› Issue (1): 35-42.doi: 10.11924/j.issn.1000-6850.casb20190700382

Special Issue: 生物技术 园艺

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Bioinformatics Analysis of BvM14-STPK Gene of Sugar Beet M14 Line and Expression Analysis of Its Response to Salt Stress

Zhao Dongmei1,2, Zhang Yongxue1,2, Li Haiying1,2()   

  1. 1 Engineering Research Center of Agricultural Microbiology Technology, Ministry of Education, Heilongjiang University, Harbin 150500;
    2 Key Laboratory of Molecular Biology, College of Heilongjiang Province, School of Life Sciences, Heilongjiang University, Harbin 150080
  • Received:2019-07-04 Revised:2019-07-29 Online:2020-01-05 Published:2020-01-07
  • Contact: Li Haiying E-mail:lvzh3000@sina.com

Abstract:

We aim to study the BvM14-STPK protein kinase in response to salt stress. Sugar beet M14 line was used as material, the full length cDNA of BvM14-STPK gene was amplified by RT-PCR with specific primers. Bioinformatics was used to analyze the full-length cDNA sequence and real-time fluorescence quantitative PCR was carried out to determine the expression patterns of BvM14-STPK in the sugar beet M14 line in response to salt stress. The results of bioinformatics analysis showed that the full-length of BvM14-STPK had 1668 bp with a 1527 bp ORF, and encoded 508 amino acids. The BvM14-STPK contained a typical protein kinase conserved domain. The results of real-time fluorescence quantitative PCR showed that the BvM14-STPK gene was expressed in the leaves and roots, and the expression in leaves were higher than that of root. Under the 200 and 400 mmol/L NaCl stress, the gene showed different expression patterns in leaves and roots, indicating that the gene could answer salt stress, but the expressed quantity was different in different organs under salt stresses. This study is significant to improving quality genes of sugar beet M14 line and enhancing crop salt-stress tolerance, and could lay a foundation for further studying genetic engineering of sugar beet.

Key words: sugar beet M14 line, BvM14-STPK, bioinformatics analysis, fluorescence quantitative PCR, salt stress

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