Trichoderma longibrachiatum T6: Strains Screening with Microwave Mutagenesis and Salt-tolerance Identification

doi:10.11924/j.issn.1000-6850.casb2020-0069

Abstract

Abstract:

The aim is to obtain the high-efficiency salt tolerant Trichoderma strains. The strain of Trichoderma longibrachiatum T6 was used as the original strain. Microwave mutagenesis and NaCl solution were used to simulate the salt stress condition. The colony growth rates, sporulation and mycelium dry weight were considered as indexes to screen the optimal salt tolerant strains. The results showed that the microwave mutation had a significant effect on the growth of Trichoderma longibrachiatum T6, and the effect was different with the different time of microwave mutation. The colony diameter, mycelium dry weight and sporulation of the mutant strain were significantly higher than those of the original strain at 60 s after microwave mutation. In addition, the colony growth rate of the mutant strain was significantly faster than other treatments, and showed stronger salt tolerance than the original strain. The colony growth rate was increased by 32.98% after 72 h of culture compared with the original strain. The high-yield and high-efficiency Trichoderma strains with salt tolerance were selected and obtained in this experiment, which laid a theoretical foundation for the development and application of multifunctional Trichoderma preparation in the future.

Key words: Trichoderma longibrachiatum T6, microwave mutagenesis, screen, salt tolerance, growth rates

CLC Number:

Cheng Kaiyong, Xu Bingliang, Wu Huayu, Chen Jinghuan, Zhang Shuwu. Trichoderma longibrachiatum T6: Strains Screening with Microwave Mutagenesis and Salt-tolerance Identification[J]. Chinese Agricultural Science Bulletin, 2020, 36(33): 66-70.

Figures/Tables 4

References 26

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培养时间/h	诱变时间
培养时间/h	0 (CK)	15 s	30 s	45 s	60 s	75 s
48	5.35±0.03a	5.28±0.01a	5.20±0.02b	5.11±0.02c	5.03±0.02d	4.96±0.03d
72	7.83±0.02d	7.95±0.03c	8.03±0.02c	8.15±0.06b	8.27±0.03a	7.93±0.02c
96	9.00±0.00a	9.00±0.00a	9.00±0.00a	9.00±0.00a	9.00±0.00a	9.00±0.00a

培养时间/h	诱变时间
培养时间/h	0 (CK)	15 s	30 s	45 s	60 s	75 s
48	5.35±0.03a	5.28±0.01a	5.20±0.02b	5.11±0.02c	5.03±0.02d	4.96±0.03d
72	7.83±0.02d	7.95±0.03c	8.03±0.02c	8.15±0.06b	8.27±0.03a	7.93±0.02c
96	9.00±0.00a	9.00±0.00a	9.00±0.00a	9.00±0.00a	9.00±0.00a	9.00±0.00a

培养时间/h	NaCl浓度/(mg/mL)	诱变时间
培养时间/h	NaCl浓度/(mg/mL)	0 s (CK)	15 s	30 s	45 s	60 s	75 s
72	0	7.80±0.06b	7.79±0.02b	7.82±0.01b	8.13±0.01a	8.20±0.03a	8.16±0.01a
	20	6.05±0.01b	6.05±0.03b	6.00±0.12b	5.10±0.03c	6.60±0.03a	5.10±0.04c
	30	3.83±0.02d	3.87±0.03d	3.89±0.03d	5.10±0.06c	6.40±0.09a	5.85±0.03b
	40	3.13±0.02d	3.15±0.03d	3.25±0.05d	4.13±0.02c	5.40±0.09a	4.51±0.03b
	50	2.47±0.01cd	2.45±0.03cd	2.49±0.04c	2.92±0.01b	3.10±0.06a	2.36±0.03d
	60	2.32±0.03b	2.35±0.01b	2.32±0.02b	2.04±0.03d	2.75±0.03a	2.23±0.03c
96	0	9.00±0.00a	9.00±0.00a	9.00±0.00a	9.00±0.00a	9.00±0.00a	9.00±0.00a
	20	9.00±0.00a	8.13±0.02c	9.00±0.00a	8.56±0.04b	9.00±0.00a	9.00±0.00a
	30	9.00±0.00a	6.75±0.02c	9.00±0.00a	7.88±0.04b	9.00±0.00a	9.00±0.00a
	40	4.65±0.02c	4.90±0.04b	4.13±0.03e	4.50±0.02d	9.00±0.00a	4.08±0.06e
	50	2.90±0.06d	3.80±0.07a	3.15±0.03c	3.47±0.03b	3.87±0.04a	2.82±0.01d
	60	2.62±0.04c	3.08±0.03a	2.70±0.06c	2.59±0.01c	3.15±0.03a	2.93±0.07b
120	0	9.00±0.00a	9.00±0.00a	9.00±0.00a	9.00±0.00a	9.00±0.00a	9.00±0.00a
	20	9.00±0.00a	9.00±0.00a	9.00±0.00a	9.00±0.00a	9.00±0.00a	9.00±0.00a
	30	9.00±0.00a	7.90±0.02b	9.00±0.00a	9.00±0.00a	9.00±0.00a	9.00±0.00a
	40	6.25±0.02b	5.90±0.06c	5.50±0.06d	4.10±0.01f	9.00±0.00a	4.92±0.03e
	50	3.30±0.06d	3.75±0.03b	3.67±0.04bc	3.62±0.04bc	4.55±0.02a	3.60±0.06c
	60	3.48±0.04c	3.70±0.05b	3.29±0.03d	3.52±0.02c	3.85±0.02a	3.31±0.05d

培养时间/h	NaCl浓度/(mg/mL)	诱变时间
培养时间/h	NaCl浓度/(mg/mL)	0 s (CK)	15 s	30 s	45 s	60 s	75 s
72	0	7.80±0.06b	7.79±0.02b	7.82±0.01b	8.13±0.01a	8.20±0.03a	8.16±0.01a
	20	6.05±0.01b	6.05±0.03b	6.00±0.12b	5.10±0.03c	6.60±0.03a	5.10±0.04c
	30	3.83±0.02d	3.87±0.03d	3.89±0.03d	5.10±0.06c	6.40±0.09a	5.85±0.03b
	40	3.13±0.02d	3.15±0.03d	3.25±0.05d	4.13±0.02c	5.40±0.09a	4.51±0.03b
	50	2.47±0.01cd	2.45±0.03cd	2.49±0.04c	2.92±0.01b	3.10±0.06a	2.36±0.03d
	60	2.32±0.03b	2.35±0.01b	2.32±0.02b	2.04±0.03d	2.75±0.03a	2.23±0.03c
96	0	9.00±0.00a	9.00±0.00a	9.00±0.00a	9.00±0.00a	9.00±0.00a	9.00±0.00a
	20	9.00±0.00a	8.13±0.02c	9.00±0.00a	8.56±0.04b	9.00±0.00a	9.00±0.00a
	30	9.00±0.00a	6.75±0.02c	9.00±0.00a	7.88±0.04b	9.00±0.00a	9.00±0.00a
	40	4.65±0.02c	4.90±0.04b	4.13±0.03e	4.50±0.02d	9.00±0.00a	4.08±0.06e
	50	2.90±0.06d	3.80±0.07a	3.15±0.03c	3.47±0.03b	3.87±0.04a	2.82±0.01d
	60	2.62±0.04c	3.08±0.03a	2.70±0.06c	2.59±0.01c	3.15±0.03a	2.93±0.07b
120	0	9.00±0.00a	9.00±0.00a	9.00±0.00a	9.00±0.00a	9.00±0.00a	9.00±0.00a
	20	9.00±0.00a	9.00±0.00a	9.00±0.00a	9.00±0.00a	9.00±0.00a	9.00±0.00a
	30	9.00±0.00a	7.90±0.02b	9.00±0.00a	9.00±0.00a	9.00±0.00a	9.00±0.00a
	40	6.25±0.02b	5.90±0.06c	5.50±0.06d	4.10±0.01f	9.00±0.00a	4.92±0.03e
	50	3.30±0.06d	3.75±0.03b	3.67±0.04bc	3.62±0.04bc	4.55±0.02a	3.60±0.06c
	60	3.48±0.04c	3.70±0.05b	3.29±0.03d	3.52±0.02c	3.85±0.02a	3.31±0.05d