Breeding of Rice Line ‘Heidaxiang1’: Combinning Molecular Marker Identification with Field Trait Selection

doi:10.11924/j.issn.1000-6850.casb2020-0760

Abstract

Abstract:

The purpose of this study is to cultivate new rice varieties with characteristics of high yield, good quality and middle maturing date, which are urgently needed in good taste rice production in Heilongjiang Province. Rice varieties meeting requirements of the breeding objectives were bred with the combination of molecular marker and field trait selection from generations of ‘Jijing88’× the introgression line ‘Daohuaxiang2(hd2hd4)’. ‘Jijing88’ had the traits of erected plant type, lodging resistance, high yield, Hd2Hd2Hd4Hd4 genotype of heading date and late maturing, and the introgression line ‘Daohuaxiang2(hd2hd4)’ possessed the characteristics of fragrance, good palatability, hd2hd2hd4hd4 genotype and early maturing. A new rice line, which was hd2hd2Hd4Hd4fgrfgr genotype, with 13 leaves of main stem, erected stem-leaf, lodging resistance, high yield, fragrance and good palatability, was bred based on offspring materials of the combination of ‘Jijing 88’×‘Daohuaxiang2(hd2hd4)’. It was named ‘Heidaxiang1’. The rice ‘Heidaxiang1’ with better agronomic characteristics could be used for good taste rice production in the lower limit of the first accumulated temperate region of Heilongjiang Province.

Key words: rice, functional molecular marker, heading date gene, fragrance gene, field trait, good taste rice production

CLC Number:

S511.22

Li Rongtian, Ma Tengxu, Wang Haoran, Liu Changhua. Breeding of Rice Line ‘Heidaxiang1’: Combinning Molecular Marker Identification with Field Trait Selection[J]. Chinese Agricultural Science Bulletin, 2021, 37(12): 1-9.

Figures/Tables 11

References 27

[1]	Emily E. Rice by the numbers: A good grain[J]. Nature, 2014,514(7524):S50-1. doi: 10.1038/514S50a URL
[2]	唐庆春, 赵立欣. 2019年黑龙江省稻米市场形势分析及后市展望[J]. 农业展望, 2020,16(04):3-6.
[3]	孙连双, 张淑华. 黑龙江省水稻育种成就、存在的问题及对策[J]. 农业开发与装备, 2013(05):68-69.
[4]	王滨. 哈尔滨:中国优质稻米之都[J]. 黑龙江粮食, 2018(07):52-55.
[5]	Masayuki M, Yumena I, Hue N T, et al. Changes in NMR Relaxation Times, Gene Expression and Quality of Seeds: Response to Different Temperature Treatments before and after the Heading Stage of Rice Plants[J]. Cryobiology and Cryotechnology, 2013,59(2):149-155.
[6]	Li X F, Liu H Z, Wang M Q, et al. Combinations of Hd2 and Hd4 genes determine rice adaptability to Heilongjiang Province, northern limit of China[J]. Journal of integrative plant biology, 2015,57(8):698-707. doi: 10.1111/jipb.v57.8 URL
[7]	李秀峰. 黑龙江省水稻抽穗期基因的效应研究及育种应用[D]. 哈尔滨:中国科学院研究生院(东北地理与农业生态研究所),2016.
[8]	孙宇琪, 卜庆云, 程云清, 等. 中国东北地区水稻抽穗期性状研究进展[J]. 土壤与作物, 2018,7(02):177-183.
[9]	崔月. 水稻抽穗期基因组合对产量相关性状的影响及其优化调控[D]. 沈阳:沈阳农业大学, 2020.
[10]	Chen S H, Yang Y, Shi W W, et al. Badh2, Encoding Betaine Aldehyde Dehydrogenase, Inhibits the Biosynjournal of 2-Acetyl-1-Pyrroline, a Major Component in Rice Fragrance(W)[J]. Plant Cell, 2008,20(7).
[11]	Buttery R G, Ling L C, Juliano B O, et al. Cooked rice aroma and 2-acetyl-1-pyrroline[J]. Journal of Agricultural and Food Chemistry, 1983,31(4):823-826. doi: 10.1021/jf00118a036 URL
[12]	彭波, 孙艳芳, 陈报阳, 等. 水稻香味基因及其在育种中的应用研究进展[J]. 植物学报, 2017,52(06):797-807.
[13]	闫影, 诸光明, 张丽霞, 等. 水稻香味基因分子标记的开发及应用[J]. 西北植物学报, 2015,35(02):269-274.
[14]	张启发. 绿色超级稻培育的设想[J]. 分子植物育种, 2005,3(5):601-602.
[15]	闫素红, 刘颖, 朱昀, 等. 抗旱基因8981转化‘吉粳88’的研究[J]. 河北农业大学学报, 2016,39(02):112-116.
[16]	李荣华, 夏岩石, 刘顺枝, 等. 改进的CTAB提取植物DNA方法[J]. 实验室研究与探索, 2009,28(09):14-16.
[17]	Seko H. Studies on lodging in rice plants[J]. Bulletin of the Kyushu Agricultural Experiment Station, 1962,7:419-499.
[18]	李荣田, 王梦露, 苏迪, 等. 利用分子标记选择培育早粳稻软米空育131(Wx-mq)导入系[J/OL].分子植物育种:1-10[2020-12-09]. http://kns.cnki.net/kcms/detail/46.1068.s.20191121.1106.003.html.
[19]	李荣田, 孟德鑫, 苏迪, 等. 利用分子标记辅助选择回交转育培育早粳稻空育131(fgr)导入系[J]. 种子, 2020,39(05):5-12.
[20]	周文甲, 田晓杰, 任月坤, 等. 利用CRISPR/Cas9创造早熟香味水稻[J]. 土壤与作物, 2017,6(02):146-152.
[21]	薛为亚, 邢永忠, 翁小煜, 等. Ghd7自然变异是调控水稻抽穗期和产量潜力的重要因素[J]. 中国基础科学, 2009,11(02):21-23.
[22]	桑茂鹏, 姜明松, 李广贤, 等. 利用分子标记辅助选择双基因Xa21和fgr水稻植株[J].山东农业科学, 2009(01):4-7.
[23]	张洪熙, 戴正元, 黄年生, 等. 长江中下游稻区籼型水稻理想株型的田间选择方法[P].中国专利:CN101317547,2008-12-10.
[24]	李扬. 基于标记辅助选择(MAS)改良粒长的水稻优异种质创制研究[D]. 上海:上海海洋大学, 2015.
[25]	王笑见. 抗倒伏和抗稻瘟病或抗褐飞虱水稻恢复系的创建[D]. 武汉:华中农业大学, 2014.
[26]	裘烨. 利用分子标记辅助选择改良恢复系稻瘟病抗性的研究[D]. 长沙:湖南农业大学, 2018.
[27]	闫影, 张丽霞, 胡泽军, 等. 利用分子标记辅助选育早熟优质香稻新品种‘沪早香软1号’[J]. 分子植物育种, 2019,17(22):7408-7413.

标记	引物	内切酶	期望条带/bp
Hd2-Caps-F	5'GGTGCGGTACCAGACCAGAAAGAGGC -3'	XcmI	84(Hd2), 101(hd2)
Hd2-Caps-R	5'CACCACCCTGCTGTTGTTGG -3'	XcmI	84(Hd2), 101(hd2)
Hd4-Caps-F	5'CGGCGCCCCGGCGCCACCGGTGCTC -3'	XhoI	84(Hd4), 104(hd4)
Hd4-Caps-R	5'GCGGCGGGTAGTCATCGAAC -3'	XhoI	84(Hd4), 104(hd4)
Fgr-SNP P₁	5'-ATTAGGTTCTGAAGCCGGTG-3'	-	440和279(Fgr), 440和188(fgr)
Fgr-SNP P₂	5'-CTGGTAAAAAGATTATGGCTTCA-3'	-
Fgr-SNP P₃	3'-ATATAAAGTCGACGAGGATAC-5'	-
Fgr-SNP P₄	3'-CAGGATATCACCACAAACTA-5'	-

标记	引物	内切酶	期望条带/bp
Hd2-Caps-F	5'GGTGCGGTACCAGACCAGAAAGAGGC -3'	XcmI	84(Hd2), 101(hd2)
Hd2-Caps-R	5'CACCACCCTGCTGTTGTTGG -3'	XcmI	84(Hd2), 101(hd2)
Hd4-Caps-F	5'CGGCGCCCCGGCGCCACCGGTGCTC -3'	XhoI	84(Hd4), 104(hd4)
Hd4-Caps-R	5'GCGGCGGGTAGTCATCGAAC -3'	XhoI	84(Hd4), 104(hd4)
Fgr-SNP P₁	5'-ATTAGGTTCTGAAGCCGGTG-3'	-	440和279(Fgr), 440和188(fgr)
Fgr-SNP P₂	5'-CTGGTAAAAAGATTATGGCTTCA-3'	-
Fgr-SNP P₃	3'-ATATAAAGTCGACGAGGATAC-5'	-
Fgr-SNP P₄	3'-CAGGATATCACCACAAACTA-5'	-

性状	吉粳88	稻花香2号(hd2hd4)
主茎剑叶与茎基角(°)	24.60±0.42	29.13±0.62^**
主茎剑叶与茎张角(°)	35.85±0.34	43.38±0.82^**
主茎剑叶与茎披垂角(°)	11.24±0.56	14.25±1.21^**
倒伏指数	149.93±1.09	174.28±1.51^*
结实率(%)	90.49±0.95	80.19±0.77^**
千粒重(g)	23.02±0.34	27.95±0.32^**
长宽比	1.23±0.02	2.71±0.01^**
叶瘟(级)	5.20±0.45	5.60±0.55
穗颈瘟(级)	5.40±0.55	5.80±0.45

性状	吉粳88	稻花香2号(hd2hd4)
主茎剑叶与茎基角(°)	24.60±0.42	29.13±0.62^**
主茎剑叶与茎张角(°)	35.85±0.34	43.38±0.82^**
主茎剑叶与茎披垂角(°)	11.24±0.56	14.25±1.21^**
倒伏指数	149.93±1.09	174.28±1.51^*
结实率(%)	90.49±0.95	80.19±0.77^**
千粒重(g)	23.02±0.34	27.95±0.32^**
长宽比	1.23±0.02	2.71±0.01^**
叶瘟(级)	5.20±0.45	5.60±0.55
穗颈瘟(级)	5.40±0.55	5.80±0.45

分子基因型			分子选择植株(株)	田间选择植株(株)	决选植株(株)	编号
Hd2(hd2)	Hd4(hd4)	Fgr(fgr)	分子选择植株(株)	田间选择植株(株)	决选植株(株)	编号
+-	++	+-	8	3	1	F₂-1
+-	+-	+-	15	5	2	F₂-2 ;F₂-3
+-	+-	--	9	2	1	F₂-4
--	+-	+-	9	3	1	F₂-5