Prothioconazole and Imazalil Mixtures: Co-toxicity and Field Control Efficacy on Fungus of Rice Bakanae Disease

doi:10.11924/j.issn.1000-6850.casb2021-0008

Abstract

Abstract:

To explore the co-toxicity and field control efficacy of the mixtures of prothioconazole and imazalil on rice bakanae disease, and develop a new reagent for controlling rice bakanae disease, Fusarium fujikuroi was used as material in this study. The toxicity of prothioconazole, imazalil and their seven mixtures on F. fujikuroi was detected by using mycelium growth rate method, and field control tests with the optimum formulation were conducted. The results showed that the synergistic coefficient was 1.9778 when the mixture ratio of prothioconazole and imazalil was 1:1, the synergistic effect was the most significant. In the field control efficacy test, the control efficacy of 500 dilution of 5% prothioconazole·imazalil WS at seedling stage, tillering stage and heading stage were 91.08%, 92.85%, and 89.52%, respectively, which were higher than that of 1000 dilution of 4.23% metalaxyl·ipconazole ME or 1000 dilution of 12% fluazinam·tebuconazole·cartap WS, and all treatments were safe to the seed germination and seedling formation of rice. In conclusion, 5% prothioconazole·imazalil WS could provide excellent control efficacy on rice bakanae disease and is safe for rice growth, which can be developed as a rice bakanae disease control agent.

Key words: rice bakanae disease, prothioconazole, imazalil, co-toxicity, control efficacy

CLC Number:

S435.111

Chen Hongzhou, Yang Zhixiang, Zhang Xinjian, Yang Hongfu, Xu Chao, Miao Kang, Chen Yuan, Yao Kebing. Prothioconazole and Imazalil Mixtures: Co-toxicity and Field Control Efficacy on Fungus of Rice Bakanae Disease[J]. Chinese Agricultural Science Bulletin, 2021, 37(14): 158-164.

Figures/Tables 4

References 23

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药剂	药剂浓度/(μg/mL)		处理菌落直径/mm								直径均值/mm		平均抑菌率/%
药剂	药剂浓度/(μg/mL)		重复1		重复2		重复3		重复4		直径均值/mm		平均抑菌率/%
丙硫菌唑	0.25		5×5		5×6		5×5		5×6		5.25		97.62
	0.125		12×12		11×12		12×12		11×12		11.75		85.24
	0.0625		25×25		25×26		25×25		25×26		25.25		59.52
	0.03125		36×36		36×37		36×36		36×37		36.25		38.57
	0.015625		42×42		42×43		42×42		42×43		42.25		27.14
抑霉唑	0.25		20×20		20×21		20×20		20×21		20.25		69.05
	0.125		28×28		28×29		28×28		28×29		28.25		53.81
	0.0625		34×35		35×35		34×35		35×35		34.75		41.43
	0.03125		38×38		38×39		38×38		38×39		38.25		34.76
	0.015625		45×46		46×46		45×46		46×46		45.75		20.48
丙硫菌唑:抑霉唑(4:1)		0.25		5×6		6×6		5×6		6×6		5.75	96.67
		0.125		10×11		10×10		10×11		10×11		10.38	87.86
		0.0625		21×22		21×21		21×22		21×22		21.38	66.90
		0.03125		32×33		33×33		32×33		33×33		32.75	45.24
		0.015625		37×38		37×37		37×38		37×38		37.38	36.43
丙硫菌唑:抑霉唑(3:1)		0.25		6×6		6×7		6×6		6×7		6.25	95.71
		0.125		12×12		12×12		12×12		12×13		12.13	84.52
		0.0625		22×22		22×23		22×22		22×23		22.25	65.24
		0.03125		31×32		32×32		32×32		32×32		31.88	46.90
		0.015625		37×37		37×37		36×37		36×37		36.75	37.62
丙硫菌唑:抑霉唑(2:1)		0.25		6×6		6×7		7×7		7×7		6.63	95.00
		0.125		10×11		11×11		11×11		11×11		10.88	86.90
		0.0625		20×20		20×20		20×20		20×21		20.13	69.29
		0.03125		30×31		30×30		30×31		31×31		30.50	49.52
		0.015625		36×36		36×37		36×37		36×36		36.25	38.57
丙硫菌唑:抑霉唑(1:1)		0.25		6×7		7×7		6×7		7×7		6.75	94.76
		0.125		11×11		11×12		11×11		11×12		11.25	86.19
		0.0625		20×20		20×21		20×20		20×21		20.25	69.05
		0.03125		30×30		30×31		30×30		30×31		30.25	50.00
		0.015625		35×36		36×36		35×36		36×36		35.75	39.52
丙硫菌唑:抑霉唑(1:2)		0.25		7×8		8×8		7×8		8×8		7.75	92.86
		0.125		12×12		12×13		12×12		12×13		12.25	84.29
		0.0625		22×23		22×22		22×23		22×23		22.38	65.00
		0.03125		34×34		33×34		34×34		34×34		33.88	43.10
		0.015625		38×39		39×39		38×39		39×39		38.75	33.81
丙硫菌唑:抑霉唑(1:3)		0.25		8×8		8×8		8×8		8×9		8.13	92.14
		0.125		13×14		14×14		13×14		14×14		13.75	81.43
		0.0625		23×23		23×24		23×23		23×24		23.25	63.33
		0.03125		33×33		33×34		33×33		33×34		33.25	44.29
		0.015625		39×39		39×39		39×39		39×40		39.13	33.10
丙硫菌唑:抑霉唑(1:4)		0.25		8×9		9×9		8×9		9×9		8.75	90.95
		0.125		15×15		15×15		15×15		15×16		15.13	78.81
		0.0625		25×25		25×25		25×25		25×25		25.00	60.00
		0.03125		35×35		35×35		35×35		35×35		35.00	40.95
		0.015625		40×40		40×41		40×41		41×41		40.50	30.48
CK		0		56×57		56×56		56×57		56×58		56.50

药剂	药剂浓度/(μg/mL)		处理菌落直径/mm								直径均值/mm		平均抑菌率/%
药剂	药剂浓度/(μg/mL)		重复1		重复2		重复3		重复4		直径均值/mm		平均抑菌率/%
丙硫菌唑	0.25		5×5		5×6		5×5		5×6		5.25		97.62
	0.125		12×12		11×12		12×12		11×12		11.75		85.24
	0.0625		25×25		25×26		25×25		25×26		25.25		59.52
	0.03125		36×36		36×37		36×36		36×37		36.25		38.57
	0.015625		42×42		42×43		42×42		42×43		42.25		27.14
抑霉唑	0.25		20×20		20×21		20×20		20×21		20.25		69.05
	0.125		28×28		28×29		28×28		28×29		28.25		53.81
	0.0625		34×35		35×35		34×35		35×35		34.75		41.43
	0.03125		38×38		38×39		38×38		38×39		38.25		34.76
	0.015625		45×46		46×46		45×46		46×46		45.75		20.48
丙硫菌唑:抑霉唑(4:1)		0.25		5×6		6×6		5×6		6×6		5.75	96.67
		0.125		10×11		10×10		10×11		10×11		10.38	87.86
		0.0625		21×22		21×21		21×22		21×22		21.38	66.90
		0.03125		32×33		33×33		32×33		33×33		32.75	45.24
		0.015625		37×38		37×37		37×38		37×38		37.38	36.43
丙硫菌唑:抑霉唑(3:1)		0.25		6×6		6×7		6×6		6×7		6.25	95.71
		0.125		12×12		12×12		12×12		12×13		12.13	84.52
		0.0625		22×22		22×23		22×22		22×23		22.25	65.24
		0.03125		31×32		32×32		32×32		32×32		31.88	46.90
		0.015625		37×37		37×37		36×37		36×37		36.75	37.62
丙硫菌唑:抑霉唑(2:1)		0.25		6×6		6×7		7×7		7×7		6.63	95.00
		0.125		10×11		11×11		11×11		11×11		10.88	86.90
		0.0625		20×20		20×20		20×20		20×21		20.13	69.29
		0.03125		30×31		30×30		30×31		31×31		30.50	49.52
		0.015625		36×36		36×37		36×37		36×36		36.25	38.57
丙硫菌唑:抑霉唑(1:1)		0.25		6×7		7×7		6×7		7×7		6.75	94.76
		0.125		11×11		11×12		11×11		11×12		11.25	86.19
		0.0625		20×20		20×21		20×20		20×21		20.25	69.05
		0.03125		30×30		30×31		30×30		30×31		30.25	50.00
		0.015625		35×36		36×36		35×36		36×36		35.75	39.52
丙硫菌唑:抑霉唑(1:2)		0.25		7×8		8×8		7×8		8×8		7.75	92.86
		0.125		12×12		12×13		12×12		12×13		12.25	84.29
		0.0625		22×23		22×22		22×23		22×23		22.38	65.00
		0.03125		34×34		33×34		34×34		34×34		33.88	43.10
		0.015625		38×39		39×39		38×39		39×39		38.75	33.81
丙硫菌唑:抑霉唑(1:3)		0.25		8×8		8×8		8×8		8×9		8.13	92.14
		0.125		13×14		14×14		13×14		14×14		13.75	81.43
		0.0625		23×23		23×24		23×23		23×24		23.25	63.33
		0.03125		33×33		33×34		33×33		33×34		33.25	44.29
		0.015625		39×39		39×39		39×39		39×40		39.13	33.10
丙硫菌唑:抑霉唑(1:4)		0.25		8×9		9×9		8×9		9×9		8.75	90.95
		0.125		15×15		15×15		15×15		15×16		15.13	78.81
		0.0625		25×25		25×25		25×25		25×25		25.00	60.00
		0.03125		35×35		35×35		35×35		35×35		35.00	40.95
		0.015625		40×40		40×41		40×41		41×41		40.50	30.48
CK		0		56×57		56×56		56×57		56×58		56.50

药剂	回归方程(y=)	相关系数(r)	EC₅₀(Eob)(95%置信限)/(μg/mL)	EC₅₀(Eth)/(μg/mL)	增效系数(SR)
丙硫菌唑	8.0804+2.1647x	0.9813	0.0378(0.0296-0.0482)
抑霉唑	6.0845+1.0402x	0.9923	0.0907(0.0782-0.1051)
丙硫菌唑:抑霉唑(4:1)	7.8547+1.8770x	0.9855	0.0301(0.0238-0.0382)	0.0428	1.4219
丙硫菌唑:抑霉唑(3:1)	7.6108+1.7144x	0.9842	0.0300(0.0234-0.0385)	0.0443	1.4767
丙硫菌唑:抑霉唑(2:1)	7.5954+1.6625x	0.9929	0.0275(0.0231-0.0327)	0.0469	1.7055
丙硫菌唑:抑霉唑(1:1)	7.5343+1.6160x	0.9923	0.0270(0.0225-0.0324)	0.0534	1.9778
丙硫菌唑:抑霉唑(1:2)	7.4317+1.6432x	0.9919	0.0331(0.0280-0.0392)	0.0618	1.8671
丙硫菌唑:抑霉唑(1:3)	7.3100+1.5749x	0.9946	0.0341(0.0298-0.0391)	0.0672	1.9707
丙硫菌唑:抑霉唑(1:4)	7.2204+1.5697x	0.9938	0.0385(0.0335-0.0442)	0.0709	1.8416

药剂	回归方程(y=)	相关系数(r)	EC₅₀(Eob)(95%置信限)/(μg/mL)	EC₅₀(Eth)/(μg/mL)	增效系数(SR)
丙硫菌唑	8.0804+2.1647x	0.9813	0.0378(0.0296-0.0482)
抑霉唑	6.0845+1.0402x	0.9923	0.0907(0.0782-0.1051)
丙硫菌唑:抑霉唑(4:1)	7.8547+1.8770x	0.9855	0.0301(0.0238-0.0382)	0.0428	1.4219
丙硫菌唑:抑霉唑(3:1)	7.6108+1.7144x	0.9842	0.0300(0.0234-0.0385)	0.0443	1.4767
丙硫菌唑:抑霉唑(2:1)	7.5954+1.6625x	0.9929	0.0275(0.0231-0.0327)	0.0469	1.7055
丙硫菌唑:抑霉唑(1:1)	7.5343+1.6160x	0.9923	0.0270(0.0225-0.0324)	0.0534	1.9778
丙硫菌唑:抑霉唑(1:2)	7.4317+1.6432x	0.9919	0.0331(0.0280-0.0392)	0.0618	1.8671
丙硫菌唑:抑霉唑(1:3)	7.3100+1.5749x	0.9946	0.0341(0.0298-0.0391)	0.0672	1.9707
丙硫菌唑:抑霉唑(1:4)	7.2204+1.5697x	0.9938	0.0385(0.0335-0.0442)	0.0709	1.8416

处理	发芽率/ %	出苗时间	成苗率/ %	苗后7d				苗后15d
处理	发芽率/ %	出苗时间	成苗率/ %	株高/m	主根长/cm	茎叶鲜重/g	根重/g	株高/cm	主根长/cm	茎叶鲜重/g	根重/g
5%丙硫菌唑·抑霉唑 WS 500倍液	81.33 a	3 d	76.50 a	10.93 c	2.96 a	0.95 b	0.48 a	16.70 b	4.33 a	3.96 a	1.52 a
5%丙硫菌唑·抑霉唑 WS 1000倍液	81.00 a	3 d	77.67 a	11.93 ab	3.07 a	1.14 a	0.49 a	17.42 ab	4.47 a	4.04 a	1.54 a
5%丙硫菌唑·抑霉唑 WS 2000倍液	82.00 a	3 d	77.33 a	11.97 ab	3.10 a	1.17 a	0.49 a	17.90 a	4.40 a	4.11 a	1.55 a
12%氟啶·戊·杀螟 WS 1000倍液	82.33 a	3 d	76.67 a	11.95 ab	3.11 a	1.14 a	0.50 a	17.25 ab	4.41 a	4.05 a	1.56 a
4.23%甲霜·种菌唑 ME 1000倍液	82.00 a	3 d	77.50 a	11.62 b	3.09 a	1.13 a	0.49 a	17.27 ab	4.50 a	4.03 a	1.54 a
CK	82.00 a	3 d	74.17 b	12.08 a	3.10 a	1.19 a	0.50 a	17.67 a	4.45 a	4.10 a	1.57 a