Welcome to Chinese Agricultural Science Bulletin,

Chinese Agricultural Science Bulletin ›› 2021, Vol. 37 ›› Issue (16): 74-81.doi: 10.11924/j.issn.1000-6850.casb2020-0457

Special Issue: 资源与环境 油料作物 水稻 农业生态

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Effect of Rice-rapeseed Annual Tillage Patterns on Soil Microecology

Gao Dujuan1(), Liu Xinglu2, Lan Zhibin2, Zhao Yang1, Chen Youde1, Zhou Bin1, Lv Yanmei1, Luo Xianfu1, Tang Shanjun1()   

  1. 1Key Laboratory of Indica Rice Genetics and Breeding in the Middle and Lower Reaches of the Yangtze River Valley,Ministry of Agriculture/ Hunan Rice Research Institute, Changsha 410125
    2Anren Agricultural Science Research Institute, Chenzhou Hunan 423600
  • Received:2020-09-12 Revised:2020-10-27 Online:2021-06-05 Published:2021-06-16
  • Contact: Tang Shanjun E-mail:gaodujuan221@163.com;shanjunt@126.com

Abstract:

To explore the response mechanism of soil microecology to rice-rapeseed system annual tillage patterns, a rice-rapeseed rotation experiment was conducted on the long-term no-tillage direct seeding soil of Dongting Lake area of north Hunan. The differences of soil enzyme activity, microorganism quantity, diversity and microbial community function between the double no-tillage direct seeding (DNT) and double rotary-tillage transplantation (DT) were compared to illustrate the effects of different tillage patterns on soil microecology. The metagenomic sequencing showed that there were similar bacterial types under the two patterns, the bacterial diversity was higher under DNT, but the fungal diversity under DT was higher. Function analysis illustrated that the relative abundance of aerobic and facultative anaerobic prokaryotes was higher in DNT; the relative abundance of prokaryotes related to nitrogen metabolism was higher in DNT, while the relative abundance of prokaryotes related to carbon metabolism was higher in DT. The number of culturable bacteria, fungi, actinomycetes and nitrifying bacteria in DNT soil was significantly lower than that in DT, while the number of ammonifying bacteria was significantly increased. Enzyme activity analysis showed that urease activity of DNT was 58.12% higher than that of DT, while the activities of invertase, phosphatase and cellulase decreased by 28.57%, 11.00% and 57.73%, respectively. The results indicated that the DNT rice-rapeseed rotation system was beneficial to improve the bacteria diversity, and the relative abundance of aerobic and facultative anaerobic prokaryotes, and was conductive to the transformation and utilization of nitrogen in the soil. The study provides a basis for the selection of reasonable tillage patterns of rice-rapeseed system in Dongting Lake area.

Key words: no-tillage, direct seeding, soil microorganism, soil enzyme activity, metagenomic sequencing, tillage pattern

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