A Novel Low Temperature Cellulose-degrading Strain Streptomyces azureus and Its Enzymatic Production Condition Optimization

doi:10.11924/j.issn.1000-6850.casb2021-0107

Abstract

Abstract:

To screen an actinomycete which can efficiently degrade cellulose in low temperature composting, and improve the degradation rate of cellulose under low temperature, a strain from litter layer of mixed forest of Korean pine was screened with Congo red staining (hydrolysis circle method) and 3,5-Dinitrosalicylic acid (DNS) method. The physiological, biochemical and molecular identification of the strain was carried out. The results showed that a strain which could degrade cellulose effectively at low temperature were named T23-B, identified as Streptomyces Azureus, and could grow at 15℃. Response surface method was performed to optimize production condition of cellulose as mannose of 15.6 g/L, yeast extract of 7.8 g/L, initial pH of 5.2, culture time of 96 h, shaking speed of 160 r/min, and culture temperature of 35℃. The cellulase activity could reach 123.43 U/mL which was 4.4 times higher than that before the optimization. Therefore, this study provides a theoretical and practical basis for applying T23-B to winter compost fermentation. It is of great significance for improve the utilization of agricultural waste resources and protect the ecological environment in cold region.

Key words: cellulose degrading bacteria, low temperature bacteria, composting, cellulase, condition optimization

CLC Number:

Li Ting, Wang Yue, Liu Zhongshan, Liu Qi, Xu Henan, Li Chongwei. A Novel Low Temperature Cellulose-degrading Strain Streptomyces azureus and Its Enzymatic Production Condition Optimization[J]. Chinese Agricultural Science Bulletin, 2021, 37(32): 25-33.

Figures/Tables 9

References 32

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ID	D/d	纤维素酶活力/(U/mL)
T13-3T	4.64±0.22b	21.12
N1-6L	3.00±0.16e	11.47
T23-B	5.40±0.37a	28.34
T13-5	4.20±0.24c	18.45
T23-3	3.73±0.19d	21.67
N4-3L	3.20±0.26e	14.32
T23-3L	3.00±0.21e	15.67

ID	D/d	纤维素酶活力/(U/mL)
T13-3T	4.64±0.22b	21.12
N1-6L	3.00±0.16e	11.47
T23-B	5.40±0.37a	28.34
T13-5	4.20±0.24c	18.45
T23-3	3.73±0.19d	21.67
N4-3L	3.20±0.26e	14.32
T23-3L	3.00±0.21e	15.67

生理生化特性	结果	生理生化特性	结果
革兰氏染色	+	硫化氢	-
糖酵解	-	甲基红	-
三糖解	+	靛基质	+
尿素酶	+	淀粉水解	+
明胶液化	+	Vp实验	+
触酶	-

生理生化特性	结果	生理生化特性	结果
革兰氏染色	+	硫化氢	-
糖酵解	-	甲基红	-
三糖解	+	靛基质	+
尿素酶	+	淀粉水解	+
明胶液化	+	Vp实验	+
触酶	-

试验序号	A.pH	B.氮源浓度/(g/mL)	C.碳源浓度/(g/mL)	纤维素酶活力/(U/mL)
1	0	0	0	124.12±1.12
2	0	1	1	103.71±0.89
3	1	0	1	112.43±0.76
4	-1	-1	0	97.85±0.98
5	0	0	0	120.19±0.83
6	0	-1	-1	105.17±0.40
7	-1	0	1	101.49±0.91
8	0	1	-1	80.08±0.62
9	1	1	0	93.27±0.76
10	1	-1	0	112.19±0.89
11	-1	0	-1	86.39±0.33
12	1	0	-1	95.19±1.09
13	0	0	0	119.14±0.82
14	0	0	0	118.33±1.46
15	-1	1	0	87.27±0.93
16	0	0	0	120.74±0.88
17	0	-1	1	108.98±1.07