Chlorophyll Fluorescence Characteristics of Lilium spp. Cultivars Under Natural High Temperature

doi:10.11924/j.issn.1000-6850.casb2021-0920

Abstract

Abstract:

A study on the response of chlorophyll fluorescence parameters of different Lilium spp. cultivars to natural high temperature was carried out to analyze the important indicators reflecting the characteristics of chlorophyll fluorescence, establish a suitable method for evaluating the high temperature response of Lilium spp. cultivars, and select sensitive and insensitive cultivars to natural high temperature. 66 Lilium spp. cultivars were selected as experimental materials, and the Fv/Fm values of functional leaves were measured under natural high temperature in Shanghai. The fuzzy membership function analysis method and the sample optimal segmentation clustering method were adopted to evaluate the heat tolerance. Under natural high temperature conditions, the chlorophyll fluorescence characteristic parameters including Fo, Fm, Fv/Fo, Fv/Fm, PI_ABS, DIo/CSo and φ_Eo in leaves were determined by rapid fluorescence detector. Then, we compared the sensitivity of different Lilium spp. cultivars. Results showed that the Fv/Fm values of the 66 cultivars were all influenced by natural high temperature. The sample optimal segmentation clustering method was used to classify the 66 cultivars into five grades. 25 cultivars, including ‘Souvenir’, ‘Merlet’, ‘Conca D'Or’, ‘Sunny Martinique’, ‘Methone’ and so on, belonged to grade I, which were not sensitive to high temperature and had strong adaptability. 19 cultivars, including ‘Zambesi’, ‘Triumphator’, ‘Nashville’, ‘Brindisi’, ‘Tresor’, etc., belonged to grade V, which were more sensitive to high temperature and had poor adaptability. This study screened and identified sensitive and insensitive Lilium spp. cultivars to high temperature, which could provide reference for further research on heat-resistant mechanism of Lilium spp. and breeding heat-resistant cultivars.

Key words: Lilium spp., chlorophyll fluorescence parameters, natural high temperature response, membership function method

CLC Number:

S682.29

CHEN Minmin, NIE Gongping, LI Xin, ZHOU Lin, YANG Liuyan, ZHANG Yongchun. Chlorophyll Fluorescence Characteristics of Lilium spp. Cultivars Under Natural High Temperature[J]. Chinese Agricultural Science Bulletin, 2022, 38(29): 86-95.

Figures/Tables 5

References 29

[1]	刘雪凝, 杨利平. 热激锻炼对亚洲百合耐热性的诱导[J]. 东北林业大学学报, 2011, 39(5):64-66.
[2]	李蕊, 杨利平, 刘雪凝. 百合杂交种及亲本耐热性比较[J]. 中国农业科学, 2011, 44(6):1201-1209.
[3]	刘艳妮, 佘奎军, 王飞. 百合耐热突变体的筛选及生理特性的研究[J]. 西北农业学报, 2012, 21(12):131-137.
[4]	张铭顺, 吕福堂, 褚鹏飞, 等. 外源Ca²⁺提高百合耐热性的生理机制初探[J]. 河南农业科学, 2019, 48(2):120-125.
[5]	XIN H, ZHANG H, CHEN L, et al. Cloning and characterization of HsfA2 from Lily (Lilium longiflorum)[J]. Plant Cell Reports, 2010, 29(8):875-885. doi: 10.1007/s00299-010-0873-1 URL
[6]	WU Z, LIANG J, ZHANG S, et al. A canonical DREB2-type transcription factor in lily is post-translationally regulated and mediates heat stress response[J]. Frontiers in plant science, 2018, 9:243 doi: 10.3389/fpls.2018.00243 pmid: 29568302
[7]	WU Z, LIANG J, WANG C, et al. Overexpression of lily HsfA3s in Arabidopsis confers increased thermo tolerance and salt sensitivity via alterations in proline catabolism[J]. Journal of experimental botany, 2018, 69(8):2005-2021. doi: 10.1093/jxb/ery035 URL
[8]	张佳平, 李丹青, 聂晶晶, 等. 高温胁迫下芍药的生理生化响应和耐热性评价[J]. 核农学报, 2016, 30(9):1848-1856. doi: 10.11869/j.issn.100-8551.2016.09.1848
[9]	王海珍, 韩路, 徐雅丽, 等. 胡杨异形叶叶绿素荧光特性对高温的响应[J]. 生态学报, 2011, 31(9):2444-2453.
[10]	郭连旺, 许大全. 自然条件下珊瑚树叶片光合作用的光抑制[J]. 植物生理学报, 1994, 20(1):46-54.
[11]	王春萍, 张世才, 雷开荣, 等. 辣椒苗期耐低氮指标与评价方法研究[J]. 园艺学报, 2017, 44(12):2318-2326.
[12]	BITA Ce, GERATS T. Plant tolerance to high temperature in a changing environment: scientific fundamentals and production of heat stress-tolerant crops[J]. Frontiers in plant science, 2013, 4:273. doi: 10.3389/fpls.2013.00273 pmid: 23914193
[13]	DERYNG D, CoNway D, RAMANKUTTY N, et al. Global crop yield response to extreme heat stress under multiple climate change futures[J]. Environmental Research Letters, 2014, 9(3):034011. doi: 10.1088/1748-9326/9/3/034011 URL
[14]	GONG B, YI J, WU J, et al. LlHSFA1, a novel heat stress transcription factor in lily (Lilium longiflorum), can interact with LiHSFA2 and enhance the thermotolerance of transgenic Arabidopsis thaliana[J]. Plant cell reports, 2014, 33(9):1519-1533. doi: 10.1007/s00299-014-1635-2 URL
[15]	CHEN Hj, TSAI Yj, SHEN Cy, et al. Ectopic expression of sweet potato cysteine protease SPCP3 alters phenotypic traits and enhances drought stress sensitivity in transgenic Arabidopsis plants[J]. Journal of plant growth regulation, 2013, 32(1):108-121. doi: 10.1007/s00344-012-9281-9 URL
[16]	KUMAR S, KAUSHAL N, NAYYAR H, et al. Abscisic acid induces heat tolerance in chickpea (Cicer arietinum L.) seedlings by facilitated accumulation of osmoprotectants[J]. Acta physiologiae plantarum, 2012, 34(5):1651-1658. doi: 10.1007/s11738-012-0959-1 URL
[17]	RAPACZ M. Chlorophyll a fluorescence transient during freezing and recovery in winter wheat[J]. Photosynthetica, 2007, 45(3): 409-418. doi: 10.1007/s11099-007-0069-2 URL
[18]	张永平, 陈幼源, 杨少军, 等. 外源水杨酸对镉胁迫甜瓜幼苗生长与光合气体交换和叶绿素荧光特性的影响[J]. 西北植物学报, 2014, 34(4):778-785.
[19]	ZOU M, YUAN L, ZHU S, et al. Effects of heat stress on photosynthetic characteristics and chloroplast ultrastructure of a heat-sensitive and heat-tolerant cultivar of wucai (Brassica campestris L.)[J]. Acta Physiologiae Plantarum, 2017, 39(1):30. doi: 10.1007/s11738-016-2319-z URL
[20]	PENG X, TENG L, YAN X, et al. The cold responsive mechanism of the paper mulberry: decreased photosynthesis capacity and increased starch accumulation[J]. BMC genomics, 2015, 16(1): 898. doi: 10.1186/s12864-015-2047-6 URL
[21]	ZHANG X, WANG W, WANG M, et al. The miR396b of Poncirus trifoliata functions in cold tolerance by regulating ACC oxidase gene expression and modulating ethylene-polyamine homeostasis[J]. Plant and cell physiology, 2016, 57(9):1865-1878. doi: 10.1093/pcp/pcw108 URL
[22]	CHEN M, THELEN J J. ACYL-LIPID DESATURASE2 is required for chilling and freezing tolerance in Arabidopsis[J]. Plant cell, 2013, 25(4):1430-1444. doi: 10.1105/tpc.113.111179 URL
[23]	查倩, 奚晓军, 蒋爱丽, 等. 高温胁迫对葡萄幼树叶绿素荧光特性和抗氧化酶活性的影响[J]. 植物生理学报, 2016, 52(4): 525-532.
[24]	吴久赟, 廉苇佳, 刘志刚, 等. 不同葡萄品种叶绿素荧光参数的高温响应及其耐热性评价[J]. 西北农林科技大学学报(自然科学版), 2019, 47(6):80-88.
[25]	徐新翔, 侯昕, 王芬, 等. 低钾胁迫对苹果砧木M9T337幼苗光合荧光特性及¹³C吸收分配的影响[J]. 园艺学报, 2020, 47(3):529-540.
[26]	张鹏, 周泉, 黄国勤. 冬季不同种植模式对稻田土壤团聚体及其有机碳的影响[J]. 核农学报, 2019, 33(12):2430-2438. doi: 10.11869/j.issn.100-8551.2019.12.2430
[27]	俞华先, 田春艳, 经艳芬, 等. 水分胁迫对4份含大茎野生种57NG208血缘F₂代甘蔗材料叶绿素荧光动力参数的影响[J]. 中国农学通报, 2019, 35(28):17-24.
[28]	杨卫丽, 黄福灯, 曹珍珍, 等. 高温胁迫对水稻光合PSII系统伤害及其与叶绿体D1蛋白间关系[J]. 作物学报, 2013, 39(6):1060-1068.
[29]	龙雅宜, 张金政, 张兰年. 百合-球根花卉之王[M]. 北京: 金盾出版社, 1999:116.

品种名称	综合值	排名	高温敏感性	品种名称	综合值	排名	高温敏感性
‘Tresor’(A)	1.4066	1	五级	‘Eyeliner’(LA)	3.8110	34	三级
‘Brindisi’(LA)	1.6664	2	五级	‘Siberia’(O)	3.8452	35	三级
‘Nashville’(LA)	1.6714	3	五级	‘Tiny Bee’(A)	3.8796	36	三级
‘Triumphator’(LO)	1.7744	4	五级	‘Roselily Samantha’(O)	3.8968	37	三级
‘Zambesi’(OT)	2.1912	5	五级	‘Roselily Thalita’(O)	3.9371	38	二级
‘Flamington’(LA)	2.3738	6	五级	‘Roselily Monica’(O)	3.9862	39	二级
‘Passion Moon’(OT)	2.3963	7	五级	‘Pearl Frances’(A)	4.0013	40	二级
‘Tiny Invader’(A)	2.4822	8	五级	‘Sheer Blonde’(O)	4.2821	41	二级
‘White Triumph’(LO)	2.4858	9	五级	‘Marlon’(O)	4.2859	42	一级
‘Pink Flight’(A)	2.5170	10	五级	‘Viviana’(O)	4.3091	43	一级
‘Indian Summerset’(LA)	2.5231	11	五级	‘Roselily Isabella’(O)	4.3680	44	一级
‘Roselily Natalia’(O)	2.5852	12	五级	‘Sorbonne’(O)	4.4076	45	一级
‘Tiny Pearl’(A)	2.5893	13	五级	‘Heartstrings’(LA)	4.4386	46	一级
‘Beau Soleil’(LA)	2.6099	14	五级	‘Roselily Elena’(O)	4.4781	47	一级
‘Honesty’(LA)	2.7644	15	五级	‘Sunny Azores’(O)	4.5070	48	一级
‘Levi’(A)	2.8284	16	五级	‘Elodie’(A)	4.5196	49	一级
‘Black Beauty’(OT)	2.8640	17	五级	‘Reeleeze’(O)	4.5551	50	一级
‘Pavia’(LA)	2.8872	18	五级	‘Matrix’(A)	4.5749	51	一级
‘Robina’(OT)	3.0079	19	五级	‘Golden Matrix’(A)	4.6138	52	一级
‘Royal Sunset’(LA)	3.0458	20	四级	‘Fangio’(LA)	4.6195	53	一级
‘Tiny Padhye’(A)	3.0796	21	四级	‘Armandale’(LA)	4.6640	54	一级
‘After Eight’(O)	3.0837	22	四级	‘Entertainer’(O)	4.7031	55	一级
‘Fata Morgana’(A)	3.0876	23	四级	‘Pink Giant’(A)	4.7428	56	一级
‘Bonsoir’(LA)	3.1161	24	四级	‘Lancifolium Flore Pleno’(A)	4.7602	57	一级
‘Trendy Havana’(A)	3.4114	25	四级	‘Love Story’(O)	4.7917	58	一级
‘White Twinkle’(A)	3.4337	26	四级	‘Manissa’(OT)	4.8399	59	一级
‘Fusion’(LL)	3.6338	27	四级	‘Dynamix’(LA)	4.9260	60	一级
‘Sunny Keys’(O)	3.6428	28	四级	‘Profundo’(OT)	4.9470	61	一级
‘Albufeira’(LA)	3.6439	29	三级	‘Methone’(LA)	4.9699	62	一级
‘Tiny Double You’(A)	3.6549	30	三级	‘Sunny Martinique’(O)	5.0770	63	一级
‘Tiny Rocket’(A)	3.7242	31	三级	‘Conca D'Or’(OT)	5.2959	64	一级
‘Mynnou’(LA)	3.7868	32	三级	‘Merlet’(LA)	5.3668	65	一级
‘Palazzo’(OT)	3.7918	33	三级	‘Souvenir’(O)	5.4386	66	一级

品种名称	综合值	排名	高温敏感性	品种名称	综合值	排名	高温敏感性
‘Tresor’(A)	1.4066	1	五级	‘Eyeliner’(LA)	3.8110	34	三级
‘Brindisi’(LA)	1.6664	2	五级	‘Siberia’(O)	3.8452	35	三级
‘Nashville’(LA)	1.6714	3	五级	‘Tiny Bee’(A)	3.8796	36	三级
‘Triumphator’(LO)	1.7744	4	五级	‘Roselily Samantha’(O)	3.8968	37	三级
‘Zambesi’(OT)	2.1912	5	五级	‘Roselily Thalita’(O)	3.9371	38	二级
‘Flamington’(LA)	2.3738	6	五级	‘Roselily Monica’(O)	3.9862	39	二级
‘Passion Moon’(OT)	2.3963	7	五级	‘Pearl Frances’(A)	4.0013	40	二级
‘Tiny Invader’(A)	2.4822	8	五级	‘Sheer Blonde’(O)	4.2821	41	二级
‘White Triumph’(LO)	2.4858	9	五级	‘Marlon’(O)	4.2859	42	一级
‘Pink Flight’(A)	2.5170	10	五级	‘Viviana’(O)	4.3091	43	一级
‘Indian Summerset’(LA)	2.5231	11	五级	‘Roselily Isabella’(O)	4.3680	44	一级
‘Roselily Natalia’(O)	2.5852	12	五级	‘Sorbonne’(O)	4.4076	45	一级
‘Tiny Pearl’(A)	2.5893	13	五级	‘Heartstrings’(LA)	4.4386	46	一级
‘Beau Soleil’(LA)	2.6099	14	五级	‘Roselily Elena’(O)	4.4781	47	一级
‘Honesty’(LA)	2.7644	15	五级	‘Sunny Azores’(O)	4.5070	48	一级
‘Levi’(A)	2.8284	16	五级	‘Elodie’(A)	4.5196	49	一级
‘Black Beauty’(OT)	2.8640	17	五级	‘Reeleeze’(O)	4.5551	50	一级
‘Pavia’(LA)	2.8872	18	五级	‘Matrix’(A)	4.5749	51	一级
‘Robina’(OT)	3.0079	19	五级	‘Golden Matrix’(A)	4.6138	52	一级
‘Royal Sunset’(LA)	3.0458	20	四级	‘Fangio’(LA)	4.6195	53	一级
‘Tiny Padhye’(A)	3.0796	21	四级	‘Armandale’(LA)	4.6640	54	一级
‘After Eight’(O)	3.0837	22	四级	‘Entertainer’(O)	4.7031	55	一级
‘Fata Morgana’(A)	3.0876	23	四级	‘Pink Giant’(A)	4.7428	56	一级
‘Bonsoir’(LA)	3.1161	24	四级	‘Lancifolium Flore Pleno’(A)	4.7602	57	一级
‘Trendy Havana’(A)	3.4114	25	四级	‘Love Story’(O)	4.7917	58	一级
‘White Twinkle’(A)	3.4337	26	四级	‘Manissa’(OT)	4.8399	59	一级
‘Fusion’(LL)	3.6338	27	四级	‘Dynamix’(LA)	4.9260	60	一级
‘Sunny Keys’(O)	3.6428	28	四级	‘Profundo’(OT)	4.9470	61	一级
‘Albufeira’(LA)	3.6439	29	三级	‘Methone’(LA)	4.9699	62	一级
‘Tiny Double You’(A)	3.6549	30	三级	‘Sunny Martinique’(O)	5.0770	63	一级
‘Tiny Rocket’(A)	3.7242	31	三级	‘Conca D'Or’(OT)	5.2959	64	一级
‘Mynnou’(LA)	3.7868	32	三级	‘Merlet’(LA)	5.3668	65	一级
‘Palazzo’(OT)	3.7918	33	三级	‘Souvenir’(O)	5.4386	66	一级

分类数	误差函数	最优分割结果
2	24.4669	1-28,29-66
3	21.0838	1-19,20-28,29-66
4	18.3857	1-31,32-37,38-41,42-66
5	15.6848	1-19,20-28,29-37,38-41,42-66
6	13.2633	1-7,8-19,20-28,29-37,38-41,42-66
7	11.2720	1-7,8-19,20-21,22-31,32-37,38-41,42-66
8	9.5398	1-19,20-28,29-37,38-41,42-58,59-62,63-66
10	6.3690	1-5,6-12,13-19,20-21,22-31,32-37,38-41,42-58,59-62,63-66
12	4.9840	1-5,6-12,13-19,20-21,22-28,29-32,33-37,38-40, 41-46, 47-58, 59-62, 63-66

分类数	误差函数	最优分割结果
2	24.4669	1-28,29-66
3	21.0838	1-19,20-28,29-66
4	18.3857	1-31,32-37,38-41,42-66
5	15.6848	1-19,20-28,29-37,38-41,42-66
6	13.2633	1-7,8-19,20-28,29-37,38-41,42-66
7	11.2720	1-7,8-19,20-21,22-31,32-37,38-41,42-66
8	9.5398	1-19,20-28,29-37,38-41,42-58,59-62,63-66
10	6.3690	1-5,6-12,13-19,20-21,22-31,32-37,38-41,42-58,59-62,63-66
12	4.9840	1-5,6-12,13-19,20-21,22-28,29-32,33-37,38-40, 41-46, 47-58, 59-62, 63-66