Abstract:

The optimal combination in tissue culture of Saintpantia ionantha was discussed in this study. Stem and axillaries of S. ionantha were used as explant for tissue culture, the basic culture medium included CC, N6, MS and B5, and major elements, organics and trace elements were selected as three different impact factors, the different concentrations of 6-BA and NAA were used to induce lateral bud, then different concentrations of combinations of NAA、IAA and IBA were used to induce root. The results show that: the optimal culture medium combination for S. ionantha is MS major elements + CC trace elements + B5 organics; the optimal combination and the concentrations for proliferating lateral bud is MS major elements + CC trace elements + B5 organics+0.5-1.0 mg/L 6-BA + 0-0.1 mg/L NAA; and the optimal culture medium combination and the concentrations for inducing root is 1/2MS+0.1mg/L NAA or 1/2MS+0.1 mg/L IAA.

Key words: Saintpantia ionantha, basic culture medium, lateral bud proliferation, rooting culture