CBD and CBDA Contents in Fermented Cannabis sativa L.: Study of Dynamic Changes

doi:10.11924/j.issn.1000-6850.casb2022-0993

Abstract

Abstract:

The aim is to study the dynamic changes of CBD and CBDA contents of Cannabis sativa L. after fermentation, clarify the correlation between CBD and CBDA, and provide reference for efficient production of CBD raw materials. With CBD and CBDA as indexes, Cannabis sativa L. were fermented by Saccharomyces cerevisiae, Lactobacillus plantarum, Escherichia coli and endophytic bacterium WF17, respectively. The dynamic changes of CBD and CBDA contents of Cannabis sativa L. before and after fermentation were analyzed by HPLC method. The CBD content reached the maximum value (3.1161 mg/g) on the first day after the fermentation of Cannabis sativa L. by Saccharomyces cerevisiae. The CBD content reached the maximum value (3.7786 mg/g) on the 9th day after Lactobacillus plantarum fermentation of Cannabis sativa L.. The content of CBD reached the maximum value (3.5502 mg/g) at 3 d after fermentation of Cannabis sativa L. by Escherichia coli. After the fermentation of Cannabis sativa L. by endophytic bacteria WF17, the CBD content reached the maximum value (3.9182 mg/g) at the fermentation time of 7 days. The content of CBDA decreased the most in the first day of fermentation. All the four fermentation strains could significantly increase the CBD content and decrease the CBDA content in the fermented Cannabis sativa L. to a certain extent, and there might be a process of CBDA to CBD in the fermentation process of Cannabis sativa L.. Therefore, fermentation technology can be used for efficient production of CBD in Cannabis sativa L..

Key words: Cannabis sativa L., cannabidiol, cannabidiolic, fermentation, HPLC

GUAN Xin, QI Kexiang, LI Wanru, ZHANG He, JIANG Shuo, WU Tong, ZHENG Chunying. CBD and CBDA Contents in Fermented Cannabis sativa L.: Study of Dynamic Changes[J]. Chinese Agricultural Science Bulletin, 2023, 39(21): 144-150.

Figures/Tables 13

References 27

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仪器	型号规格	生产厂家
电子精密天平	PL303	梅特勒-托利多仪器有限公司
超净工作台	SZX	南通科学仪器有限公司
空气浴振荡器	HZQ-C	哈尔滨市东联电子技术开发有限公司
旋转蒸发仪	R-201	上海申胜生物技术有限公司
超声波清洗器	KQ-100DE	昆山市超声仪器有限公司
紫外可见波长检测器	FL2000	浙江温岭福立分析仪器有限公司
高效液相色谱仪	FL2000	浙江温岭福立分析仪器有限公司
400MH固体超导核磁共振波谱仪	AVANCE Ⅲ	美国Thermo Fisher公司

仪器	型号规格	生产厂家
电子精密天平	PL303	梅特勒-托利多仪器有限公司
超净工作台	SZX	南通科学仪器有限公司
空气浴振荡器	HZQ-C	哈尔滨市东联电子技术开发有限公司
旋转蒸发仪	R-201	上海申胜生物技术有限公司
超声波清洗器	KQ-100DE	昆山市超声仪器有限公司
紫外可见波长检测器	FL2000	浙江温岭福立分析仪器有限公司
高效液相色谱仪	FL2000	浙江温岭福立分析仪器有限公司
400MH固体超导核磁共振波谱仪	AVANCE Ⅲ	美国Thermo Fisher公司

参数	设置条件
色谱柱	Venusil XBP-C18柱(4.6 mm×250 mm，5 μm，USA)
流动相及波长	①号流动相：乙腈-0.1%甲酸水溶液(75:25)，220 nm； ②号流动相：甲醇-乙腈-0.1%磷酸水溶液(40:30:30)，254 nm
流速	1 mL/min
柱温	25℃
进样量	10 μL

参数	设置条件
色谱柱	Venusil XBP-C18柱(4.6 mm×250 mm，5 μm，USA)
流动相及波长	①号流动相：乙腈-0.1%甲酸水溶液(75:25)，220 nm； ②号流动相：甲醇-乙腈-0.1%磷酸水溶液(40:30:30)，254 nm
流速	1 mL/min
柱温	25℃
进样量	10 μL

组分	回归方程	相关系数r	线性范围/(mg/mL)
CBD	Y=6×10⁶X+264479	0.9998	0.0625~1.00
CBDA	Y=8.5198×10⁵X+47651	0.9998	0.0625~1.00