Regulation Effect of StGID1 Gene on Potato Plant Height, Diameter and Chlorophyll Content

doi:10.11924/j.issn.1000-6850.casb2023-0472

Abstract

Abstract:

In order to investigate the role of gibberellin insensitive dwarf gene (GID1) in potato growth, the gene was cloned and transformed to record the expression differences. StGID1 was cloned from ‘Chuanyu 21’ tissue culture seedlings by using the primers designed from PGSC (Potato Genomics Resource). By transformation into potato ‘E Malingshu 3’, overexpressing transgenic plants and RNAi plants were obtained, and the differences in expression were recorded. Phylogenetic analysis showed that the amino acid sequence of StGID1 protein had the highest homology with SlGID1b-1 in tomato. The comparison of overexpression transgenic plants and suppressed expression transgenic plants showed that in the tissue culture seedlings, the average stem diameter of suppressed expression transgenic plants was twice that of overexpression plants. In the mature plants, the relative height of the suppressed expression plants was only 62.8% of that in the overexpressed plants, significantly lower than the blank control and overexpressed plants. When treated with gibberellin, it showed an increase in stem height and a decrease in stem diameter in overexpression plants, suppressed expression plants, and control, with increasing of gibberellin concentration. This study verified that StGID1 was a homologous gene of GID1 gene family in potato and was a part of the gibberellin regulation pathway in potato.

Key words: Solanum tuberosum, StGID1, phylogenetic analysis, gibberellin regulation pathway

LI Huapeng, PENG Xiaohe, LIANG Xiao, TANG Mingxia, WANG Kexiu, HU Jianjun. Regulation Effect of StGID1 Gene on Potato Plant Height, Diameter and Chlorophyll Content[J]. Chinese Agricultural Science Bulletin, 2024, 40(15): 102-109.

Figures/Tables 14

References 19

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引物	序列(5’-3’)
21OV49-F	ACGGGGGACTCTAGAGGATCCATGGTGGACACTAAAGAGA
21OV49-R	CGATCGGGGAAATTCGAGCTCCTAGGAATGGTTAGGATGTAT
21OV49-NF	ACGGGGGACTCTAGAGGATCCATGCAGAGGGTGGTTCCA
21OV49-NR	CGATCGGGGAAATTCGAGCTCCTAGGAATGGTTAGGATGTAT
35S-seqF	CACTATCCTTCGCAAGACCC

引物	序列(5’-3’)
21OV49-F	ACGGGGGACTCTAGAGGATCCATGGTGGACACTAAAGAGA
21OV49-R	CGATCGGGGAAATTCGAGCTCCTAGGAATGGTTAGGATGTAT
21OV49-NF	ACGGGGGACTCTAGAGGATCCATGCAGAGGGTGGTTCCA
21OV49-NR	CGATCGGGGAAATTCGAGCTCCTAGGAATGGTTAGGATGTAT
35S-seqF	CACTATCCTTCGCAAGACCC

引物	序列(5’-3’)
21RI05-F1	TTTGGAGAGGACACGCTCGAGTTGGACGGGAAATACTTTGTG
21RI05-R1	TGGGGTACCGAATTCCTCGAGCCTCCATTAGGCAACGAAAAT
21RI05-F2	GATAAGCTTGGATCCTCTAGACCTCCATTAGGCAACGAAAAT
21RI05-R2	TCATTAAAGCAGGACTCTAGATTGGACGGGAAATACTTTGTG
35S-seqF	CACTATCCTTCGCAAGACCC

引物	序列(5’-3’)
21RI05-F1	TTTGGAGAGGACACGCTCGAGTTGGACGGGAAATACTTTGTG
21RI05-R1	TGGGGTACCGAATTCCTCGAGCCTCCATTAGGCAACGAAAAT
21RI05-F2	GATAAGCTTGGATCCTCTAGACCTCCATTAGGCAACGAAAAT
21RI05-R2	TCATTAAAGCAGGACTCTAGATTGGACGGGAAATACTTTGTG
35S-seqF	CACTATCCTTCGCAAGACCC

21OTK01			21OTK02			E3
	茎高/mm	茎粗/mm		茎高/mm	茎粗/mm		茎高/mm	茎粗/mm
①	78.12	0.42	①	72.57	1.49	①	51.63	1.03
②	61.41	0.36	②	81.00	1.73	②	69.62	1.17
③	76.53	0.18	③	43.84	1.91	③	79.39	1.74
④	78.20	0.13	④	69.15	1.79	④	67.29	1.09
⑤	52.23	0.32	⑤	69.73	1.92	⑤	75.22	1.89
⑥	65.52	0.51	⑥	86.91	1.53	⑥	81.04	1.41
⑦	69.81	1.03	⑦	73.70	1.80	⑦	61.22	1.80
⑧	50.63	1.41	⑧	59.20	1.89	⑧	52.06	0.86
⑨	73.22	1.50	⑨	67.02	1.60	⑨	39.51	1.02
⑩	77.77	1.53	⑩	66.71	1.52	⑩	42.44	0.61
⑪	57.31	1.44	⑪	58.99	1.76	⑪	51.03	0.91
⑫	74.66	1.48	⑫	48.03	1.73	⑫	46.58	1.28
平均	67.95	0.86	平均	66.40	1.72	平均	59.75	1.23