Amomun tsao-ko Germplasm Resources in Different Origins: Cold Resistance Evaluation

doi:10.11924/j.issn.1000-6850.casb2023-0407

Abstract

Abstract:

The research aims to screen out the germplasm resource of Amomun tsao-ko with high cold resistance, provide a basis for hybrid breeding and cultivation of Amomun tsao-ko and facilitate the sustainable development of the Amomun tsao-ko industry. Taking Amomun tsao-ko plants from different origins which has been grown for 6 months as test materials, the materials were cultured under low temperature stress for 3 days (low temperature incubator parameters: 4℃, 12 hours of darkness, 12 hours of light, 90% humidity), the physiological indexes related to cold resistance were measured, and cold resistance of the Amomun tsao-ko germplasm resources of different origins was comprehensively evaluated by using correlation anailsis, principal component analysis and membership function analysis. The results showed that under low temperature (4℃) stress, the chlorophyll content and antioxidant enzyme activity in the leaves of Amomun tsao-ko decreased, while the electrical conductivity, malondialdehyde content, soluble sugar content, and soluble protein content increased to varying degrees. The rank of the cold resistance of the Amomun tsao-ko obtained by the membership function analysis is DH-LC>BS-TC>NJ-GS-GLB>WS-MG>PE-ZY>PE-LC. Among the 6 germplasms from different origins, DH-LC has the best cold resistance, and it could be used as cold resistance material for hybrid breeding and cultivation.

Key words: Amomun tsao-ko, germplasm resources, low temperature stress, cold tolerance screening, membership function analysis

ZHAO Xinyu, SU Junyu, YANG Wei, YANG Jianning, TONG Kairong, YANG Zhiqing. Amomun tsao-ko Germplasm Resources in Different Origins: Cold Resistance Evaluation[J]. Chinese Agricultural Science Bulletin, 2024, 40(15): 73-79.

Figures/Tables 6

References 18

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编号	海拔/m	采集地址
PE-LC	1336	云南省普洱市澜沧县
WS-MG	1542	云南省文山州马关县
PE-ZY	1376	云南省普洱市镇沅县勐大镇里崴乡
BS-TC	1796.06	云南省腾冲市马站乡三联村
NJ-GS-GLB	1562.2	云南省怒江州贡山县茨开镇嘎拉博村
DH-LC	1900	云南省德宏州陇川县护国乡护国村委会护国上寨

编号	海拔/m	采集地址
PE-LC	1336	云南省普洱市澜沧县
WS-MG	1542	云南省文山州马关县
PE-ZY	1376	云南省普洱市镇沅县勐大镇里崴乡
BS-TC	1796.06	云南省腾冲市马站乡三联村
NJ-GS-GLB	1562.2	云南省怒江州贡山县茨开镇嘎拉博村
DH-LC	1900	云南省德宏州陇川县护国乡护国村委会护国上寨

编号	叶绿素含量/(mg/g)	蛋白质含量/(mg/g)	可溶性糖含量/(mg/g)	淀粉含量/(μg/g)
PE-LC	1.54±0.23c	102.93±24.09a	10.01±1.81c	417.42±62.54ab
WS-MG	2.09±0.18ab	91.65±26.24a	13.92±4.1abc	386.29±112.04b
PE-ZY	1.75±0.17bc	30.07±8.77b	13.42±1.12abc	380.81±63.49b
BS-TC	2.45±0.19a	10.42±13.55b	16.66±1.47a	470.37±132.49ab
NJ-GS-GLB	2.22±0.13a	46.51±26.36b	12.12±1.67bc	591.07±139.4a
DH-LC	2.39±0.28a	33.56±15.33b	15.91±2.08ab	513.68±44.83ab

编号	叶绿素含量/(mg/g)	蛋白质含量/(mg/g)	可溶性糖含量/(mg/g)	淀粉含量/(μg/g)
PE-LC	1.54±0.23c	102.93±24.09a	10.01±1.81c	417.42±62.54ab
WS-MG	2.09±0.18ab	91.65±26.24a	13.92±4.1abc	386.29±112.04b
PE-ZY	1.75±0.17bc	30.07±8.77b	13.42±1.12abc	380.81±63.49b
BS-TC	2.45±0.19a	10.42±13.55b	16.66±1.47a	470.37±132.49ab
NJ-GS-GLB	2.22±0.13a	46.51±26.36b	12.12±1.67bc	591.07±139.4a
DH-LC	2.39±0.28a	33.56±15.33b	15.91±2.08ab	513.68±44.83ab

编号	CAT/[μmol/(min·g)]	SOD/(U/g)	POD/[△OD₄₇₀/(min·g)]	MDA/(nmol/g)
PE-LC	0.58±0.41a	839.05±941.65a	395.67±31.5a	15.29±3.36a
WS-MG	0.19±0.03b	762.98±164.38a	340.36±64.81a	11.51±2.1ab
PE-ZY	0.63±0.2a	419.77±344.84a	341.07±96.76a	10.62±2.52b
BS-TC	0.17±0.12b	723.08±853.99a	327.92±75.53a	10.47±2.49b
NJ-GS-GLB	0.06±0.05b	719.31±386.5a	303.26±57.97a	9.74±1.91b
DH-LC	0.7±0.15a	590.91±76.91a	303.91±25.88a	10.09±0.68b