Effects of Rhizophagus intraradices on Biomass of Rotational Cropping and Alternate Cropping Soybean

doi:10.11924/j.issn.1000-6850.casb2023-0868

Abstract

Abstract:

The purpose of this study was to investigate the role of Rhizophagus intraradices in alleviating alternate cropping resistance and increasing soybean biomass. The soybean variety ‘Heinong 48’ was used as experimental material. The spore density of arbuscular mycorrhizal (AM) fungi, the colonization rate of AM fungi, and the soybean biomass were determined using wet sieve decantation-sucrose centrifugation, alkali separation-acid fuchsin method and direct measurement, respectively. The results showed that the AM fungi spore density in the rhizosphere soil of rotational cropping soybean field was 2.2 times higher than that of alternate cropping soybean field. Under natural soil conditions, the AM fungal colonization rate of soybean roots and soybean biomass significantly increased after inoculation with R. intraradices in the rotational cropping and alternate cropping soil compared to the untreated group. Additionally, the root length was the most significantly increased by 31.60% and 33.57%, respectively. The soybean biomass inoculated with R. intraradices in the rotational cropping soil was similar to that of the untreated group in the alternate cropping soil. Under the same inoculation conditions, the AM fungal colonization rate of soybean roots and the soybean biomass in the sterilized soil were lower than those in the natural soil. This study laid a theoretical foundation for alleviating the obstacle of soybean alternate cropping resistance and improving soybean yield.

Key words: soybean, rotational cropping and alternate cropping, alternate cropping resistance, rhizophagus intraradices, colonization rate, biomass, arbuscular mycorrhizal fungal spore density

ZHANG Min, JIE Weiguang, TAN Yiwen, KAN Lianbao, MENG Jianxia, SUN Shanshan, ZHANG Yong, SHI He, ZHANG Siyi. Effects of Rhizophagus intraradices on Biomass of Rotational Cropping and Alternate Cropping Soybean[J]. Chinese Agricultural Science Bulletin, 2024, 40(20): 37-45.

Figures/Tables 10

References 34

[1]	林凤岩, 黄永娜, 褚洪俊, 等. 我国大豆蛋白加工产业现状及发展趋势[J]. 中国油脂, 2023, 48(11):33-37,56.
[2]	李嘉伦, 李洋洋, 姜凯岩. 氨基寡糖素用于大豆田杀菌剂、叶面肥减量[J]. 现代化农业, 2023(11):5-8.
[3]	何微, 聂迎利, 王晓梅, 等. 全球大豆种子市场现状及中国企业发展启示[J]. 农业展望, 2023, 19(9):40-45.
[4]	COLEMAN K, WHITMORE A P, HASSALL K L, et al. The potential for soybean to diversify the production of plant-based protein in the UK[J]. Science of the total environment, 2021, 767(3):144903.
[5]	JIE W G, LIN J X, GUO N, et al. Community composition of rhizosphere fungi as affected by Funneliformis mosseae in soybean continuous cropping soil during seedling period[J]. Chilean joural of agricultural research, 2019, 79(3):356-365.
[6]	ZHANG W W, FENG Z Z, WANG X K, et al. Quantification of ozone exposure and stomatal uptake-yield response relationships for soybean in Northeast China[J]. Science of the total environment, 2017,599-600:710-720.
[7]	王亚楠, 吕杰. 东北地区玉米和大豆生产成本效率的区域差异及影响因素[J]. 农业经济, 2020, 395(3):12-14.
[8]	WANG Y, XU X, LIU T, et al. Analysis of bacterial and fungal communities in continuous-cropping ramie (Boehmeria nivea L. Gaud) fields in different areas in China[J]. Scientific reports, 2020, 10(1):3564-3569.
[9]	JIE W G, TAN Y W, YANG D Y, et al. Effects of Rhizophagus intraradices and Acinetobacter calcoaceticus on soybean growth and carbendazim residue[J]. Sustainability, 2023, 15(13):10322.
[10]	KUMAWAT K C, RAZDAN N, SAHARAN K. Rhizospheric microbiome: Bio-based emerging strategies for sustainable agriculture development and future perspectives[J]. Microbiological research, 2022, 254:126901.
[11]	MUNIR N, HANIF M, ABIDEEN Z, et al. Mechanisms and strategies of plant microbiome interactions to mitigate abiotic stresses[J]. Agronomy, 2022, 12:2069.
[12]	GAI J P, FAN J Q, ZHANG S B, et al. Direct effects of soil cadmium on the growth and activity of arbuscular mycorrhizal fungi[J]. Rhizosphere, 2018, 7:43-48.
[13]	ZHAO Y, CARTABIA A, LALAYMIA I, et al. Arbuscular mycorrhizal fungi and production of secondary metabolites in medicinal plants[J]. Mycorrhiza, 2022, 32(3-4):221-256. doi: 10.1007/s00572-022-01079-0 pmid: 35556179
[14]	ULLAH U Z. REHMAN A N, ZAHOOR A, et al. Dose optimization of silicon for boosting arbuscular mycorrhizal fungi colonization and cadmium stress mitigation in maize (Zea mays L.)[J]. Environmental science and pollution research international, 2023, 30(25):67071-67086.
[15]	ZENG H L, ZHONG W, TAN F X, et al. The influence of Bt maize cultivation on communities of arbuscular mycorrhizal fungi revealed by MiSeq sequencing[J]. Frontiers in microbiology, 2019, 9:3275.
[16]	MARRO N, COFRE N, GRILLI G, et al. Soybean yield, protein content and oil quality in response to interaction of arbuscular mycorrhizal fungi and native microbial populations from mono- and rotation-cropped soils[J]. Applied soil ecology, 2020, 152:103575.
[17]	ZHANG F G, LIU M H, LI Y, et al. Effects of arbuscular mycorrhizal fungi, biochar and cadmium on the yield and element uptake of Medicago sativa[J]. Science of the total environment, 2019, 655:1150-1158.
[18]	MA X, LUO W, LI J, et al. Arbuscular mycorrhizal fungi increase both concentrations and bioavilability of Zn in wheat (Triticum aestivum L.) grain on Zn-spiked soils[J]. Applied soil ecology, 2018, 135:91-97.
[19]	吴会会, 吴强盛. 盐胁迫下丛枝菌根真菌对枳实生苗生长及生理代谢的影响[J]. 中国南方果树, 2023, 52(6):8-13.
[20]	NOROOZI N, MOHAMMADI G, GHOBADI M. Some physio-biochemical traits of sunflower as affected by arbuscular mycorrhizal fungi inoculation under different irrigation treatments[J]. Italian journal of agronomy, 2023, 18: 2033.
[21]	NAHID M, HADI K, SAEED A. Alleviation of Rhizoctonia root rot damage in common bean by some arbuscular mycorrhizal fungi[J]. Journal of applied research in plant protection, 2023, 12(1):13-24.
[22]	接伟光, 林厚泽, 杨冬莹, 等. 根内根孢囊霉对大豆成熟期生物量、根腐病及根际土壤百菌清残留的影响[J]. 江苏农业科学, 2023, 51(14):153-158.
[23]	宋福强, 程蛟, 常伟, 等. 田间施加AM菌剂对大豆生长效应的影响[J]. 中国农学通报, 2013, 29(6):69-74.
[24]	郭美玲, 郭泰, 王志新, 等. 大豆重迎茬问题及与玉米轮作大豆季整地技术综述[J]. 农业科技通讯, 2023(3):165-168.
[25]	接伟光, 杨冬莹, 姚延轩, 等. 正茬与迎茬对大豆根际土壤微生物群落组成的影响[J]. 大豆科学, 2022, 41(2):179-188.
[26]	李森, 姚钦, 刘俊杰, 等. 大豆重迎茬研究进展[J]. 大豆科学, 2020, 39(2):317-324.
[27]	李伟群. 不同年限连作大豆根际微生物群落及生物量的动态变化研究[D]. 北京: 中国农业科学院, 2011.
[28]	YANG Z, DONG H, ZHANG S, et al. Isolation and identification of mycorrhizal helper bacteria of Vaccinium uliginosum and their interaction with mycorrhizal fungi[J]. Front microbiol, 2023, 14:1-13.
[29]	SANGWAN S, PRASANNA R. Mycorrhizae helper bacteria: Unlocking their potential as bioenhancers of plant-arbuscular mycorrhizal fungal associations[J]. Microbial ecology, 2022, 84(1):1-10.
[30]	宋瑞清, 邓勋, 宋小双. 菌根辅助细菌与外生菌根互作机制研究进展[J]. 吉林农业大学学报, 2016, 36(4):379-384.
[31]	BAI L, CUI J Q, JIE W G, et al. Analysis of the community compositions of rhizosphere fungi in soybeans continuous cropping fields[J]. Microbiological research, 2015, 180:49-56. doi: 10.1016/j.micres.2015.07.007 pmid: 26505311
[32]	LI Y, SHI C, WEI D, et al. Soybean continuous cropping affects yield by changing soil chemical properties and microbial community richness[J]. Microbiol, 2022, 13:1083736.
[33]	SPAGNILETTI F N, LEIVA M, CHIOCCHIO V, et al. Phosphorus fertilization reduces the severity of charcoal rot (Macrophomina phaseolina) and the arbuscular mycorrhizal protection in soybean[J]. Journal of plant nutrition and soil science, 2018, 181:855-860.
[34]	NACOON S, JOGLOY S, RIDDECH N, et al. Combination of arbuscular mycorrhizal fungi and phosphate solubilizing bacteria on growth and production of Helianthus tuberosus under field condition[J]. Scientific reports, 2021, 11(1):6501. doi: 10.1038/s41598-021-86042-3 pmid: 33753844

土壤类型	AM真菌孢子密度/（个/g）
正茬(0y)	5.3±0.6
迎茬(1y)	2.4±0.4

土壤类型	AM真菌孢子密度/（个/g）
正茬(0y)	5.3±0.6
迎茬(1y)	2.4±0.4

处理方式	30 d	60 d	90 d	120 d
0yInN	12.05±0.98^b	38.21±1.26^a	59.48±1.58^a	90.25±2.13^a
0yNonN	5.13±0.63^c	11.09±1.02^c	20.98±1.19^c	28.73±1.56^c
0yCkN	5.20±0.75^c	10.85±0.96^c	22.06±1.33^c	26.02±1.37^c
0yInM	10.97±1.02^b	30.45±1.41^b	51.32±1.52^b	85.41±1.98^b
1yInN	14.26±1.21^a	42.19±1.52^a	64.72±1.84^a	93.53±2.35^a
1yNonN	6.92±0.56^c	13.06±0.51^c	24.47±1.19^c	32.85±1.52^c
1yCkN	6.84±0.81^c	12.91±0.87^c	26.39±1.45^c	30.34±1.47^c
1yInM	12.26±1.12^b	34.52±1.46^b	55.33±1.58^b	89.76±1.75^a

处理方式	30 d	60 d	90 d	120 d
0yInN	12.05±0.98^b	38.21±1.26^a	59.48±1.58^a	90.25±2.13^a
0yNonN	5.13±0.63^c	11.09±1.02^c	20.98±1.19^c	28.73±1.56^c
0yCkN	5.20±0.75^c	10.85±0.96^c	22.06±1.33^c	26.02±1.37^c
0yInM	10.97±1.02^b	30.45±1.41^b	51.32±1.52^b	85.41±1.98^b
1yInN	14.26±1.21^a	42.19±1.52^a	64.72±1.84^a	93.53±2.35^a
1yNonN	6.92±0.56^c	13.06±0.51^c	24.47±1.19^c	32.85±1.52^c
1yCkN	6.84±0.81^c	12.91±0.87^c	26.39±1.45^c	30.34±1.47^c
1yInM	12.26±1.12^b	34.52±1.46^b	55.33±1.58^b	89.76±1.75^a

处理方式	30 d	60 d	90 d	120 d
0yInN	7.33±0.85^a	58.13±0.95^a	87.69±1.16^a	101.12±1.23^a
0yNonN	6.23±0.93^b	52.47±1.02^c	72.57±1.09^c	87.23±1.14^c
0yCkN	6.42±0.58^b	51.56±0.78^c	74.82±0.97^c	85.82±1.03^c
0yInM	7.28±0.91^a	55.58±0.93^b	80.79±1.23^b	95.35±1.17^b
0yNonM	6.12±0.68^bc	50.42±1.02^cd	69.35±1.08^cd	80.86±1.22^d
0yCkM	6.04±0.72^bc	49.01±0.63^cd	71.92±1.64^cd	81.19±1.31^d
1yInN	6.55±0.76^b	53.29±0.75^b	76.33±0.87^c	89.27±1.09^c
1yNonN	5.85±0.68^c	48.37±0.73^d	62.25±1.12^d	71.42±1.28^de
1yCkN	5.68±0.52^c	47.63±0.65^d	61.71±1.26^d	70.33±1.41^de
1yInM	6.37±0.60^b	51.27±0.58^c	70.39±1.47^c	82.86±1.39^cd
1yNonM	5.51±0.77^c	45.33±0.49^e	58.72±1.05^e	65.39±1.51^e
1yCkM	5.31±0.69^c	44.82±0.52^e	56.99±1.03^e	64.28±1.37^e