Analysis on Population Fluctuation of Rhizosphere Microorganism of Resistant and Susceptible Bitter Gourd

doi:10.11924/j.issn.1000-6850.casb2023-0634

Abstract

Abstract:

In this study, 28 varieties of Momordica charantia were used to evaluate the resistance to Fusarium oxysporum f. sp. momordica by artificial root-soaking inoculation in seedling stage. Results showed that bitter gourd germplasms had considerable difference in Fusarium wilt-resistance, among them, 1 high resistance (HR) variety and 11 high susceptibility (HS) varieties were screened out and selected for the further analysis of population fluctuation of rhizosphere microorganism. The results showed that the correlation between the population of Fusarium oxysporum in soil and the occurrence of Fusarium wilt was different, and the incidence of Fusarium wilt was significant positively correlated with the Fusarium oxysporum. The microorganism community structure of resistant variety was better than susceptible variety. The richness and diversity of the resistant varieties rhizosphere soil microbial community, Sobs, Shannon and Chao 1 indexes of fungi were increased by 36.93%, 25.89% and 38.58% respectively, and Sobs, Shannon and Chao 1 indexes of bacteria increased by 13.63%, 12.91% and 26.52%. The content of nitrate nitrogen, available phosphorus, ammonium nitrogen, inorganic phosphorus, pH and organic matter of resistant varieties were higher than that of susceptible varieties at seedling stage, the composition of rhizosphere microorganism were affected by pH, nitrogen and available phosphorus.

Key words: Momordica charantia, Fusarium wilt, disease resistance, rhizosphere microorganism, soil physicochemical properties, biodiversity

SHI Bo, XIE Yuanyuan, GUAN Feng, YANG Xuetong, ZHANG Jingyun, WANG Kai, WAN Xinjian. Analysis on Population Fluctuation of Rhizosphere Microorganism of Resistant and Susceptible Bitter Gourd[J]. Chinese Agricultural Science Bulletin, 2024, 40(22): 118-124.

Figures/Tables 7

References 25

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序号	品种	抗性等级	发病率/%	病情指数	序号	品种	抗性等级	发病率/%	病情指数
1	BK0604	HR	0.15±0.05	3.15±0.31	15	LK1413	MR	38.45±2.55	31.04±0.69
2	BK0201	R	80.11±1.67	27.65±1.21	16	LK1520	HS	100.00±3.69	100.00±7.21
3	BK0703	S	58.33±2.10	57.36±2.78	17	BK0605	R	25.62±1.41	29.22±1.21
4	BK2001	S	90.23±5.87	66.77±0.52	18	BK1606	HS	88.60±2.57	88.46±2.11
5	BK1901	R	51.05±2.18	30.57±1.25	19	BK1713	HS	100.00±6.62	95.83±2.12
6	BK1903	HS	100.00±0.55	81.35±3.66	20	LK1721	HS	95.66±2.33	89.37±3.37
7	BK0011	R	84.66±6.78	28.56±1.12	21	LK0901	HS	100.00±1.23	100.00±3.12
8	BK0208	HS	100.00±4.36	97.25±5.81	22	BK1812	R	37.55±2.56	18.35±0.34
9	LK0009	R	50.00±1.33	22.14±0.95	23	Z1501	HS	100.00±3.23	95.66±4.98
10	BK0702	HS	100.00±6.31	97.92±6.60	24	Z1525	HS	100.00±6.21	97.25±6.45
11	BK1403	HS	100.00±2.88	100.00±4.48	25	Z1709	R	31.33±3.89	25.86±1.21
12	LK1904	S	75.00±2.36	65.98±3.57	26	BK0701	HS	100.00±3.89	100.00±2.56
13	BK1522	S	54.16±5.22	66.37±3.21	27	LK1402	R	45.89±6.21	19.38±0.35
14	LK1604	S	71.85±3.56	56.52±2.11	28	LK1109	R	83.65±6.32	24.58±0.65

序号	品种	抗性等级	发病率/%	病情指数	序号	品种	抗性等级	发病率/%	病情指数
1	BK0604	HR	0.15±0.05	3.15±0.31	15	LK1413	MR	38.45±2.55	31.04±0.69
2	BK0201	R	80.11±1.67	27.65±1.21	16	LK1520	HS	100.00±3.69	100.00±7.21
3	BK0703	S	58.33±2.10	57.36±2.78	17	BK0605	R	25.62±1.41	29.22±1.21
4	BK2001	S	90.23±5.87	66.77±0.52	18	BK1606	HS	88.60±2.57	88.46±2.11
5	BK1901	R	51.05±2.18	30.57±1.25	19	BK1713	HS	100.00±6.62	95.83±2.12
6	BK1903	HS	100.00±0.55	81.35±3.66	20	LK1721	HS	95.66±2.33	89.37±3.37
7	BK0011	R	84.66±6.78	28.56±1.12	21	LK0901	HS	100.00±1.23	100.00±3.12
8	BK0208	HS	100.00±4.36	97.25±5.81	22	BK1812	R	37.55±2.56	18.35±0.34
9	LK0009	R	50.00±1.33	22.14±0.95	23	Z1501	HS	100.00±3.23	95.66±4.98
10	BK0702	HS	100.00±6.31	97.92±6.60	24	Z1525	HS	100.00±6.21	97.25±6.45
11	BK1403	HS	100.00±2.88	100.00±4.48	25	Z1709	R	31.33±3.89	25.86±1.21
12	LK1904	S	75.00±2.36	65.98±3.57	26	BK0701	HS	100.00±3.89	100.00±2.56
13	BK1522	S	54.16±5.22	66.37±3.21	27	LK1402	R	45.89±6.21	19.38±0.35
14	LK1604	S	71.85±3.56	56.52±2.11	28	LK1109	R	83.65±6.32	24.58±0.65

品种	细菌/(×10⁶ cfu/g)	放线菌/(×10⁵cfu/g)	真菌/(×10⁴cfu/g)	尖孢镰刀菌/(×10³cfu/g)	总量/(×10⁶cfu/g)
BK0604	11.21±0.36	8.12±0.49	4.58±0.12	3.45±0.21	10.78±0.17
BK0201	7.85±0.35	6.85±0.34	5.78±0.10	3.78±0.32	7.89±0.35
BK0703	2.21±0.02	2.10±0.05	8.12±0.05	3.99±0.05	8.78±0.65
BK2001	2.36±0.15	1.56±0.09	8.78±0.12	3.78±0.63	8.44±0.96
BK1901	8.17±0.07	6.21±0.35	5.66±0.55	4.01±0.22	7.21±1.01
BK1903	1.39±0.08	1.23±0.07	12.01±1.58	3.21±0.55	1.45±1.55
BK0011	7.15±0.63	6.52±0.21	5.21±0.52	3.69±0.05	8.85±1.01
BK0208	1.52±0.10	1.21±0.05	11.25±0.69	3.47±0.26	2.78±0.56
LK0009	6.98±0.21	5.89±0.15	5.89±0.14	3.87±0.02	6.87±0.36
BK0702	1.88±0.14	1.85±0.16	11.56±0.36	3.21±0.25	1.85±0.55
BK1403	1.76±0.11	1.69±0.07	10.28±0.68	4.20±0.69	1.98±0.54
LK1904	2.55±0.09	2.15±0.11	8.05±0.21	3.65±0.08	4.15±0.22
BK1522	2.15±0.08	2.26±0.13	8.65±0.58	3.11±0.12	3.21±0.17
LK1604	2.58±0.14	1.89±0.06	8.80±0.20	3.65±0.25	3.26±0.21
LK1413	4.37±0.18	5.04±0.13	5.33±0.23	3.09±0.06	4.78±0.11
LK1520	1.69±0.21	1.21±0.01	11.69±0.36	3.36±0.21	1.58±0.28
BK0605	6.89±0.25	6.22±0.13	5.25±0.21	4.01±0.05	7.21±0.88
BK1606	1.78±0.19	1.39±0.04	12.12±0.23	3.78±0.31	1.98±0.21
BK1713	1.68±0.15	1.87±0.23	11.25±0.45	3.79±0.55	1.85±0.14
LK1721	1.69±0.08	2.05±0.08	11.36±0.08	4.23±0.96	2.01±0.05
LK0901	1.28±0.05	1.58±0.07	10.56±0.31	3.35±0.65	1.87±0.25
BK1812	7.12±0.32	6.12±0.10	5.25±0.25	3.37±0.21	5.12±0.11
Z1501	1.36±0.06	1.58±0.14	12.56±0.32	3.47±0.05	1.56±0.05
Z1525	1.85±0.11	1.08±0.02	11.25±0.17	3.98±0.52	1.68±0.15
Z1709	7.12±0.58	6.89±0.34	6.12±0.41	3.78±0.36	8.15±0.45
BK0701	0.21±0.23	2.11±0.36	11.16±0.13	3.89±0.24	1.89±0.22
LK1402	7.15±0.16	5.88±0.12	6.01±0.08	3.69±0.21	8.21±0.21
LK1109	6.42±0.51	6.08±0.13	5.89±0.31	3.78±0.19	8.89±0.35

品种	细菌/(×10⁶ cfu/g)	放线菌/(×10⁵cfu/g)	真菌/(×10⁴cfu/g)	尖孢镰刀菌/(×10³cfu/g)	总量/(×10⁶cfu/g)
BK0604	11.21±0.36	8.12±0.49	4.58±0.12	3.45±0.21	10.78±0.17
BK0201	7.85±0.35	6.85±0.34	5.78±0.10	3.78±0.32	7.89±0.35
BK0703	2.21±0.02	2.10±0.05	8.12±0.05	3.99±0.05	8.78±0.65
BK2001	2.36±0.15	1.56±0.09	8.78±0.12	3.78±0.63	8.44±0.96
BK1901	8.17±0.07	6.21±0.35	5.66±0.55	4.01±0.22	7.21±1.01
BK1903	1.39±0.08	1.23±0.07	12.01±1.58	3.21±0.55	1.45±1.55
BK0011	7.15±0.63	6.52±0.21	5.21±0.52	3.69±0.05	8.85±1.01
BK0208	1.52±0.10	1.21±0.05	11.25±0.69	3.47±0.26	2.78±0.56
LK0009	6.98±0.21	5.89±0.15	5.89±0.14	3.87±0.02	6.87±0.36
BK0702	1.88±0.14	1.85±0.16	11.56±0.36	3.21±0.25	1.85±0.55
BK1403	1.76±0.11	1.69±0.07	10.28±0.68	4.20±0.69	1.98±0.54
LK1904	2.55±0.09	2.15±0.11	8.05±0.21	3.65±0.08	4.15±0.22
BK1522	2.15±0.08	2.26±0.13	8.65±0.58	3.11±0.12	3.21±0.17
LK1604	2.58±0.14	1.89±0.06	8.80±0.20	3.65±0.25	3.26±0.21
LK1413	4.37±0.18	5.04±0.13	5.33±0.23	3.09±0.06	4.78±0.11
LK1520	1.69±0.21	1.21±0.01	11.69±0.36	3.36±0.21	1.58±0.28
BK0605	6.89±0.25	6.22±0.13	5.25±0.21	4.01±0.05	7.21±0.88
BK1606	1.78±0.19	1.39±0.04	12.12±0.23	3.78±0.31	1.98±0.21
BK1713	1.68±0.15	1.87±0.23	11.25±0.45	3.79±0.55	1.85±0.14
LK1721	1.69±0.08	2.05±0.08	11.36±0.08	4.23±0.96	2.01±0.05
LK0901	1.28±0.05	1.58±0.07	10.56±0.31	3.35±0.65	1.87±0.25
BK1812	7.12±0.32	6.12±0.10	5.25±0.25	3.37±0.21	5.12±0.11
Z1501	1.36±0.06	1.58±0.14	12.56±0.32	3.47±0.05	1.56±0.05
Z1525	1.85±0.11	1.08±0.02	11.25±0.17	3.98±0.52	1.68±0.15
Z1709	7.12±0.58	6.89±0.34	6.12±0.41	3.78±0.36	8.15±0.45
BK0701	0.21±0.23	2.11±0.36	11.16±0.13	3.89±0.24	1.89±0.22
LK1402	7.15±0.16	5.88±0.12	6.01±0.08	3.69±0.21	8.21±0.21
LK1109	6.42±0.51	6.08±0.13	5.89±0.31	3.78±0.19	8.89±0.35

	发病率	尖孢镰刀菌数量	真菌数量	细菌数量	放线菌数量
尖孢镰刀菌数量	0.778^**	1.000
真菌数量	0.879^**	0.734^**	1.000
细菌数量	-0.765^**	-0.829^**	-0.722^**	1.000
放线菌数量	-0.935^**	-0.785^**	-0.852^**	0.825^**	1.000
微生物数量	-0.737^**	-0.926^**	-0.767^**	0.998^**	0.751