Identification of Pestalotiopsis microspora Causing Fruit Spot on Guava and Laboratory Screening of Fungicides

doi:10.11924/j.issn.1000-6850.casb2023-0676

Abstract

Abstract:

In order to clarify the pathogen causing guava fruit spot disease and to control the disease, the pathogens from guava leaves and fruit with typical symptoms were collected for isolation and purification. Based on tissue isolation method, phylogenetic analysis, morphological characters and Koch’s postulates, the pathogen was identified as Pestalotiopsis microspora. Inhibitory activities of six fungicides to P. microspora Guava A, Guava B and Guava C were determined using mycelial growth method. The result indicated 450 g/L prochloraz EW had the best inhibitory effect on the mycelial growth, with an EC₅₀values of 0.0019 μg/mL; the following was 60% pyraclostrobin·fluxapyroxad SC, 75% trifloxystrobin·tebuconazole WG and 325 g/L difenoconazole·azoxystrobin SC, with EC₅₀values of 0.1479 μg/mL, 0.1697 μg/mL and 0.1739 μg/mL. The result provides a reference for the control of fruit spot disease on guava in field.

Key words: guava, leaf spot, fruit spot, Pestalotiopsis microspora, fungicide, inhibitory activity in vitro

CUI Yiping, CHEN Huiqian, SONG Xiaobing, PENG Aitian, HUANG feng, LING Jinfeng. Identification of Pestalotiopsis microspora Causing Fruit Spot on Guava and Laboratory Screening of Fungicides[J]. Chinese Agricultural Science Bulletin, 2024, 40(25): 96-102.

Figures/Tables 5

References 25

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供试杀菌剂	别名	编号	浓度/(μg/mL)	生产产家
75%肟菌·戊唑醇WG（25%肟菌酯、50%戊唑醇）	拿敌稳	A1	0.1	拜耳（中国）有限公司
		A2	0.5
		A3	2
		A4	10
		A5	50
35% 400 g/L氟菌·戊唑醇SC（17.5%氟吡菌酰胺、17.5%戊唑醇）	露娜润	B1	0.1	拜耳（中国）有限公司
		B2	0.5
		B3	2
		B4	10
		B5	50
325 g/L苯甲·嘧菌酯SC（125 g/L苯醚甲环唑、200 g/L嘧菌酯）	阿米妙收/凯典	C1	0.1	先正达集团公司
		C2	0.5
		C3	2
		C4	10
		C5	50
60%唑醚·代森联WG（5%吡唑醚菌酯、55%代森联）	百泰	D1	0.1	巴斯夫（中国）有限公司
		D2	0.5
		D3	2
		D4	10
		D5	50
10%苯醚甲环唑WG	恶醚唑	E1	0.2	先正达集团公司
		E2	0.4
		E3	0.6
		E4	0.8
		E5	1.0
450 g/L咪鲜胺EW	施保克	F1	0.2	苏州富美实植物保护剂有限公司
		F2	0.4
		F3	0.6
		F4	0.8
		F5	1.0

供试杀菌剂	别名	编号	浓度/(μg/mL)	生产产家
75%肟菌·戊唑醇WG（25%肟菌酯、50%戊唑醇）	拿敌稳	A1	0.1	拜耳（中国）有限公司
		A2	0.5
		A3	2
		A4	10
		A5	50
35% 400 g/L氟菌·戊唑醇SC（17.5%氟吡菌酰胺、17.5%戊唑醇）	露娜润	B1	0.1	拜耳（中国）有限公司
		B2	0.5
		B3	2
		B4	10
		B5	50
325 g/L苯甲·嘧菌酯SC（125 g/L苯醚甲环唑、200 g/L嘧菌酯）	阿米妙收/凯典	C1	0.1	先正达集团公司
		C2	0.5
		C3	2
		C4	10
		C5	50
60%唑醚·代森联WG（5%吡唑醚菌酯、55%代森联）	百泰	D1	0.1	巴斯夫（中国）有限公司
		D2	0.5
		D3	2
		D4	10
		D5	50
10%苯醚甲环唑WG	恶醚唑	E1	0.2	先正达集团公司
		E2	0.4
		E3	0.6
		E4	0.8
		E5	1.0
450 g/L咪鲜胺EW	施保克	F1	0.2	苏州富美实植物保护剂有限公司
		F2	0.4
		F3	0.6
		F4	0.8
		F5	1.0

供试药剂	毒力回归方程	决定系数	EC₅₀/(μg/mL)	标准误
75%肟菌·戊唑醇WG	Y=5.5115X+0.664	0.9745	0.1697	0.0291
35% 400 g/L氟菌·戊唑醇SC	Y=4.3146X+0.8358	0.9447	6.6082	0.0548
325 g/L苯甲·嘧菌酯SC	Y=5.551X+0.7253	0.9572	0.1739	0.0414
60%唑醚·代森联WG	Y=5.5366X+0.6465	0.9348	0.1479	0.0467
10%苯醚甲环唑WG	Y=4.5638X+0.0114	0.0977	243567	0.0221
450 g/L咪鲜胺EW	Y=5.693X+0.2549	0.8012	0.0019	0.0360

供试药剂	毒力回归方程	决定系数	EC₅₀/(μg/mL)	标准误
75%肟菌·戊唑醇WG	Y=5.5115X+0.664	0.9745	0.1697	0.0291
35% 400 g/L氟菌·戊唑醇SC	Y=4.3146X+0.8358	0.9447	6.6082	0.0548
325 g/L苯甲·嘧菌酯SC	Y=5.551X+0.7253	0.9572	0.1739	0.0414
60%唑醚·代森联WG	Y=5.5366X+0.6465	0.9348	0.1479	0.0467
10%苯醚甲环唑WG	Y=4.5638X+0.0114	0.0977	243567	0.0221
450 g/L咪鲜胺EW	Y=5.693X+0.2549	0.8012	0.0019	0.0360