Screening and Identification of Plant Growth Promoting Bacteria of Tussilago farfara and Its Inoculation Effect

doi:10.11924/j.issn.1000-6850.casb2024-0014

Abstract

Abstract:

Growth-promoting characteristics and effects of plant growth promoting bacteria (PGPR) strains isolated from the rhizosphere and root system of Tussilago farfara were studied, in order to provide potential strains for the high-yield cultivation of T. farfara. PGPR strains were obtained by dilution-plating method, and their growth-promoting characteristics were analyzed. These strains were identified by morphological, physiological and biochemical BIOLOG and 16s rDNA sequence. The inoculation effects of these strains on seedlings of T. farfara were examined by a pot-culture experiment. The results showed that three high-yielding IAA strains were selected from the rhizosphere soil and roots of T. farfara, named Enterobacter cloacae subsp. dissolvens (KD6B), Klebsiella oxytoca (KD17B) and K. areogenes (KD26B), which had effects of solubilize phosphorus, produce siderophore, salt tolerance, drought tolerance and antagonism. Three strains significantly increased the number of leaves, leaf area, root length and diversity index of rhizosphere soil microbial community of T. farfara seedlings. Among them, KD17B had the most obvious effect, with the number of leaves, leaf area and root length increasing by 36.36%, 29.21% and 54.07% respectively, and the average color change rate (AWCD), Shannon index and McIntosh index were the largest. Three PGPR strains have significant growth-promoting effects on T. farfara and regulate the structure of rhizosphere soil microbial community, which have high application value in the high-yield cultivation of T. farfara.

Key words: Tussilago farfara, plant growth promoting bacteria (PGPR), screening, inoculation effect, soil microbial community diversity

ZHANG Lihui, FENG Dan, BAI Bianxia, JIN Tingting, REN Jiahong. Screening and Identification of Plant Growth Promoting Bacteria of Tussilago farfara and Its Inoculation Effect[J]. Chinese Agricultural Science Bulletin, 2024, 40(34): 33-41.

Figures/Tables 16

References 32

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菌株	IAA/(mg/L)	解磷/(mg/L)	嗜铁素能力/%
KD6B	26.95±0.77b	327.0±6.71c	81.62±4.19b
KD17B	27.63±1.46b	568.8±7.32b	30.27±1.65c
KD26B	32.63±0.60a	679.8±6.66a	92.07±3.58a

菌株	IAA/(mg/L)	解磷/(mg/L)	嗜铁素能力/%
KD6B	26.95±0.77b	327.0±6.71c	81.62±4.19b
KD17B	27.63±1.46b	568.8±7.32b	30.27±1.65c
KD26B	32.63±0.60a	679.8±6.66a	92.07±3.58a

生理生化指标	KD6B	KD17B	KD26B
革兰氏染色	G^-	G^-	G^-
接触酶试验	+	+	+
KOH溶解试验	+	+	+
需氧性试验	好氧	好氧	好氧
油脂水解试验	+	-	-
淀粉水解试验	-	-	-
伏普试验	-	-	+
甲基红试验	-	+	+
吲哚试验	-	-	-
硝酸盐还原试验	-	+	+
纤维素分解试验	-	-	-
明胶水解试验	+	-	-
菌膜形成试验	+	-	-

生理生化指标	KD6B	KD17B	KD26B
革兰氏染色	G^-	G^-	G^-
接触酶试验	+	+	+
KOH溶解试验	+	+	+
需氧性试验	好氧	好氧	好氧
油脂水解试验	+	-	-
淀粉水解试验	-	-	-
伏普试验	-	-	+
甲基红试验	-	+	+
吲哚试验	-	-	-
硝酸盐还原试验	-	+	+
纤维素分解试验	-	-	-
明胶水解试验	+	-	-
菌膜形成试验	+	-	-

菌株	叶面积/cm²	根长/cm	叶片数量/片
CK	92.62±9.02d	13.5±0.42d	5.5±0.71c
KD6B	159.56±9.67a	17.2±0.28c	6.5±0.71bc
KD26B	141.64±0.51b	19.9±0.71b	8.0±1.41a
KD17B	119.67±3.63c	20.8±0.57a	7.5±0.71ab