Abstract:

Ganoderma lucidum is an excellent traditional Chinese medicine, with a huge market demand in Asia and other regions. And the traditional intensive cultivation always results in low yield and poor quality of ganoderma lucidum. Water-dry rotation has been proved to be a practical, effective and sustainable agricultural measure, but study on ganoderma lucidum-rice rotation is extremely rare. To explore soil microbial communities’ succession at different stages of ganoderma lucidum-rice rotation, high-throughput sequencing was used to analyze soil bacteria and fungal communities structure at different stages of DAF, L₁ and L₂. The results showed that from DAF to L₂, the diversity index values of bacteria gradually decreased, and that of fungi decreased and then increased. The ganoderma lucidum-rice rotation had a significant impact on the structure of bacterial and fungal communities. The relative abundance of rhodobacter and gemmatimonas at DAF was significantly higher than that at L₁ and L₂. And abundance of MND1, escherichia coli-Shigella, Dongia, streptomyces and Ellin6067 were the opposite. As for fungi, the relative abundance of psathyrella at L₁ was significantly higher than that at DAF and L₂, while mortierella was the opposite. The correlation analysis showed that most of the soil chemical nutrient indicators were significantly negatively correlated with the fungal diversity indicators, but not with the bacterial richness, bacterial diversity and fungal richness. RDA analysis showed that SOM was the main environmental factor affecting the bacterial community, while AP and AK were the main factors affecting the fungal community. Ganoderma lucidum-rice rotation is beneficial to improve the soil microbial community structure, increase the yield and quality of ganoderma lucidum. Thus, it has a significant positive effect on the sustainable agricultural production of ganoderma lucidum.

Key words: Ganoderma lucidum, rotation, community structure, correlation analysis