Pathogen Identification of Radish Black Rot and Fungicides Screening in Laboratory

doi:10.11924/j.issn.1000-6850.casb2023-0162

Abstract

Abstract:

A serious radish rot was found in the field of Tianjin. 5 bacterial isolates were isolated from diseased radish tissues. In order to provide reference for the diagnosis and prevention of this disease, pathogen identification was carried out using morphology, pathogenicity test and moleculer identification, and the toxicities of 20 kinds of bactericides were tested by inhibition zone method. The results showed that the pathogen was identified as Xanthomonas campestris pv. campestris. Antibacterial activity differed among the different agents. Among 20 kinds of the tested agents, 13 agents had antibacerial effects on Xanthomonas campestris pv. campestris, of which 0.3% tetramycin aqueous solutions and 80% ethylicin emulsifiable concentrate were more effective than 98% streptomycin sulphate TC. 7 agents, including 27.12% copper dihydroxosulphate suspension concentrate, had no inhibitory action.

Key words: radish, black rot, Xanthomonas campestris pv. campestris, fungicides, antibacterial effect

YANG Lijuan, GAO Wei, LI Haiyan, XUE Tao, ZHANG Chunxiang, WANG Yong. Pathogen Identification of Radish Black Rot and Fungicides Screening in Laboratory[J]. Chinese Agricultural Science Bulletin, 2024, 40(7): 129-134.

Figures/Tables 4

References 26

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杀菌剂	抑菌圈直径
杀菌剂	100 mg/L	200 mg/L	500 mg/L	1000 mg/L	2000 mg/L	3000 mg/L
CK	-	-	-	-	-	-
98%硫酸链霉素原药	-	-	7.8	8.0	9.0	9.7
20%松脂酸铜水乳剂	-	-	-	8.0	8.0	8.0
27.12%碱式硫酸铜悬浮剂	-	-	-	-	-	-
86.2%氧化亚铜可湿性粉剂	-	-	-	-	8.0	9.0
20%乙酸铜可湿性粉剂	-	-	-	-	7.6	8.0
20%噻森铜悬浮剂	-	-	-	-	7.0	7.0
15%络氨铜水剂	-	-	-	-	6.4	6.8
30%琥胶肥酸铜可湿性粉剂	-	-	-	-	-	8.4
10%混合氨基酸铜水剂	-	-	-	-	-	8.0
30%王铜悬浮剂	-	-	-	-	-	8.0
46%氢氧化铜水分散粒剂	-	-	-	-	-	-
77%硫酸铜钙可湿性粉剂	-	-	-	-	-	-
20%噻菌铜悬浮剂	-	-	-	-	-	-
80%代森锌水分散粒剂	-	-	-	-	-	-
70%代森联水分散粒剂	-	-	-	-	10.0	11.0
5%大蒜素微乳剂	-	-	-	-	-	-
80%乙蒜素乳油	-	-	14.6	12.0	19.4	21.4
6%低聚糖素水剂	-	-	-	-	7.4	8.4
20%春雷霉素水分散粒剂	-	-	-	-	-	-
0.3%四霉素水剂	21.0	23.0	29.4	32.0	35.4	41.0

杀菌剂	抑菌圈直径
杀菌剂	100 mg/L	200 mg/L	500 mg/L	1000 mg/L	2000 mg/L	3000 mg/L
CK	-	-	-	-	-	-
98%硫酸链霉素原药	-	-	7.8	8.0	9.0	9.7
20%松脂酸铜水乳剂	-	-	-	8.0	8.0	8.0
27.12%碱式硫酸铜悬浮剂	-	-	-	-	-	-
86.2%氧化亚铜可湿性粉剂	-	-	-	-	8.0	9.0
20%乙酸铜可湿性粉剂	-	-	-	-	7.6	8.0
20%噻森铜悬浮剂	-	-	-	-	7.0	7.0
15%络氨铜水剂	-	-	-	-	6.4	6.8
30%琥胶肥酸铜可湿性粉剂	-	-	-	-	-	8.4
10%混合氨基酸铜水剂	-	-	-	-	-	8.0
30%王铜悬浮剂	-	-	-	-	-	8.0
46%氢氧化铜水分散粒剂	-	-	-	-	-	-
77%硫酸铜钙可湿性粉剂	-	-	-	-	-	-
20%噻菌铜悬浮剂	-	-	-	-	-	-
80%代森锌水分散粒剂	-	-	-	-	-	-
70%代森联水分散粒剂	-	-	-	-	10.0	11.0
5%大蒜素微乳剂	-	-	-	-	-	-
80%乙蒜素乳油	-	-	14.6	12.0	19.4	21.4
6%低聚糖素水剂	-	-	-	-	7.4	8.4
20%春雷霉素水分散粒剂	-	-	-	-	-	-
0.3%四霉素水剂	21.0	23.0	29.4	32.0	35.4	41.0