Abstract:

To understand the characteristics and evolutionary relationships of key enzymes involved in the tyrosine metabolism pathway in sugar beets, taking plants such as sugar beet, spinach, and soybean as experimental materials, sequence information of five key enzymes in the tyrosine metabolism pathway (PPO, TAT, TYDC, HPPD, HPPR) was collected using the NCBI database. The physicochemical properties of the key enzymes were analyzed using ExPASy-ProtParam software and MEGA-7 software, their sequence similarities and evolutionary distances were compared, and a phylogenetic tree was constructed. The analysis of physicochemical properties showed that all five key enzymes in the tyrosine metabolism pathway of sugar beet were unstable hydrophilic proteins, and the number of amino acids in sugar beet PPO was the highest; the phylogenetic tree analysis results showed that there was high similarity in PPO and HPPD between sugar beet and spinach; beets had high similarity with TAT of soybeans, TYDC of potatoes, and HPPR of barley, respectively. This study provided a reference for other crops to explore the key enzyme function of sugar beet tyrosine metabolism pathway and laid a foundation for further research on sugar beet genomics.

Key words: beet, tyrosine, catalytic enzyme, metabolic pathway, bioinformatics analysis