Isolation and Identification of Pathogenic Fungi Causing Sorghum Anthracnosis in Jiangsu Province

doi:10.11924/j.issn.1000-6850.casb2024-0788

Abstract

Abstract:

To identify the pathogenic fungi causing sorghum anthracnose in Jiangsu Province, typical diseased samples were collected from three main sorghum-producing areas (Sihong, Lianshui and Haimen) during 2022-2023. Single-spore isolation was performed to obtain pathogenic strains, and Koch’s postulates were applied to verify pathogenicity. Pathogen identification was conducted using morphological characteristics and molecular methods based on ITS and GAPDH genes. The results showed that 47 single-spore strains were isolated from 15 diseased samples, and representative strains reproduced typical anthracnose symptoms upon reinoculation on sorghum. The conidia were falcate-shaped, colorless and unicellular, measuring (10-25) µm × (3.5-5) µm. The appressoria was nearly spherical and brown, consistent with the characteristics of Colletotrichum species. The ITS and GAPDH sequences exhibited over 98% and 96% homology, respectively, with Colletotrichum sublineola, and phylogenetic analysis clustered the isolates within the C. sublineola clade. This study is the first to confirm C. sublineola as the pathogen responsible for sorghum anthracnose in Jiangsu Province, providing a theoretical basis for accurate diagnosis and control of this disease.

Key words: sorghum, anthracnose, Colletotrichum sublineola, Jiangsu Province, pathogen isolation, molecular identification, pathogenicity

REN Chunmei, GUO Sisi, WANG Haitao, CHENG Zhaobang, LU Fang, YANG Liu, JI Yinghua. Isolation and Identification of Pathogenic Fungi Causing Sorghum Anthracnosis in Jiangsu Province[J]. Chinese Agricultural Science Bulletin, 2025, 41(31): 95-101.

Figures/Tables 5

References 28

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