Abstract:

This study aims to identify the C2H2 zinc finger protein gene family members in rose (Rosa chinensis) using bioinformatics approaches, and to analyze their physicochemical properties, chromosomal localization, gene structures, and expression patterns, thereby providing a foundation for understanding the functional mechanisms of C2H2 proteins. A total of 43 C2H2 genes were identified in rose, distributed across seven chromosomes and classified into five subfamilies. Synteny analysis revealed 41 collinear gene pairs between rose and Arabidopsis thaliana, and 10 collinear gene pairs within the rose genome. Conserved motif analysis identified five motifs, among which Motif 1 was the core motif. Promoter regions contained various cis-acting elements associated with abscisic acid response, anaerobic stress, photoperiod, and others. Protein interaction analysis indicated that 29 C2H2 gene family members exhibited interactions, with RcC2H2zfp7 serving as a central hub node. Tissue expression analysis revealed that members of this gene family exhibited the highest expression levels in roots, followed by stamens. This study systematically elucidates the basic characteristics and expression patterns of the C2H2 zinc finger protein gene family in rose, providing a theoretical reference for further exploration of their functions in growth, development, and stress responses.

Key words: Rosa chinensis, C2H2 gene family, bioinformatics, genome-wide identification, physicochemical properties, phylogenetic tree, cis-acting element, expression analysis