Abstract:

Persimmon is rich in natural phenolic compounds which have varieties of physiological functions. To separate and identify the polyphenols in persimmon, HP2MGL macroporous adsorption resin, Sephadex LH-20 polysaccharide gels and ODS-A reverse-phase media were employed orderly. Four different phenols in Diospyros kaki L. cv. Mopan, gallic acid, catechin, (-)-epicatechin and caffeine acid, were obtained, which were detected and identified using LC/MS method. The purity of gallic acid, catechin, (-)-epicatechin and caffeine acid was 95.15%, 95.04%, 87.47% and 85.0% respectively. An effective method of isolating and purifying phenolic components from Diospyros kaki L. cv. Mopan was established in this study.