Abstract: The study aims to establish a direct regeneration system for cultivated sugar beet in northeast China, and lay a foundation for the cultivation of sugar beet varieties by genetic engineering. In this study, cotyledonary sections and petioles were used as explants to study the effects of different disinfection methods and hormone levels on the induction of adventitious bud and adventitious root. The results showed that: the best disinfection method was stripping the seed shell to obtain seeds and the lowest pollution rate was performed using 75% alcohol for 30 s and 0.1% HgCl2 for 5 min; the optimum ratio of hormone to the induction of adventitious bud was MS+6-BA 0.5 mg/L+NAA 0.05 mg/L; the induction rate of adventitious bud using both cotyledon and petiole were biggest with 14.44% and 11.67%, respectively; the optimum ratio of hormone to induce adventitious root was 1/2MS + NAA 0.05 mg/L, and the induction rate was 93.33% . This study preliminarily establishes the direct regeneration system for sugar beet in northeast China, laying the foundation for the establishment of genetic transformation system and genetic improvement of sugar beet.